Afp 10 primer pairs found

Mouse

Show all (including non-specific)

NM_007423 · chr5

117 Specific

FWD 5' TCCTGCTACATTTCGCTGCG 3'

REV 5' TGGCTTCCGGAACAAACTGG 3'

Fwd Tm 62.6°C

Rev Tm 62.6°C

GC 55%

Product 144 bp

Spans 2 introns

NM_007423 · chr5

117 Specific

FWD 5' TCCTGCTACATTTCGCTGCG 3'

REV 5' GCTTCCGGAACAAACTGGGT 3'

Fwd Tm 62.6°C

Rev Tm 62.6°C

GC 55%

Product 142 bp

Spans 2 introns

NM_007423 · chr5

117 Specific

FWD 5' TCCTGCTACATTTCGCTGCG 3'

REV 5' GGTGGCTTCCGGAACAAACT 3'

Fwd Tm 62.6°C

Rev Tm 62.6°C

GC 55%

Product 146 bp

Spans 2 introns

NM_007423 · chr5

117 Specific

FWD 5' CTGCTACATTTCGCTGCGTC 3'

REV 5' GTGGCTTCCGGAACAAACTG 3'

Fwd Tm 61.3°C

Rev Tm 61.3°C

GC 55%

Product 143 bp

Spans 2 introns

NM_007423 · chr5

117 Specific

FWD 5' CTGCGTCCAAAGCATTGCAC 3'

REV 5' TGGCTTCCGGAACAAACTGG 3'

Fwd Tm 62.4°C

Rev Tm 62.6°C

GC 55%

Product 129 bp

Spans 2 introns

NM_007423 · chr5

117 Specific

FWD 5' CATTTCGCTGCGTCCAAAGC 3'

REV 5' TGGCTTCCGGAACAAACTGG 3'

Fwd Tm 62.4°C

Rev Tm 62.6°C

GC 55%

Product 136 bp

Spans 2 introns

NM_007423 · chr5

117 Specific

FWD 5' CTGCGTCCAAAGCATTGCAC 3'

REV 5' GCTTCCGGAACAAACTGGGT 3'

Fwd Tm 62.4°C

Rev Tm 62.6°C

GC 55%

Product 127 bp

Spans 2 introns

NM_007423 · chr5

117 Specific

FWD 5' CTGCGTCCAAAGCATTGCAC 3'

REV 5' GGTGGCTTCCGGAACAAACT 3'

Fwd Tm 62.4°C

Rev Tm 62.6°C

GC 55%

Product 131 bp

Spans 2 introns

NM_007423 · chr5

117 Specific

FWD 5' CATTTCGCTGCGTCCAAAGC 3'

REV 5' GCTTCCGGAACAAACTGGGT 3'

Fwd Tm 62.4°C

Rev Tm 62.6°C

GC 55%

Product 134 bp

Spans 2 introns

NM_007423 · chr5

117 Specific

FWD 5' CATTTCGCTGCGTCCAAAGC 3'

REV 5' GGTGGCTTCCGGAACAAACT 3'

Fwd Tm 62.4°C

Rev Tm 62.6°C

GC 55%

Product 138 bp

Spans 2 introns