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F5 NM_000130

Human hg38 · chr1 118 Specific

Primer Sequences

Forward pos: 169,525,943
5'— CCAGAGAGCAGGGATGCAAA —3'
Reverse pos: 169,523,899
5'— TTGCTAATCTGGGCTCCCAG —3'
Validate in NCBI Primer-BLAST

Primer Metrics

Forward Tm 62.0°C
Reverse Tm 61.5°C
GC 55%
Forward GC
GC 55%
Reverse GC

Community Reviews

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Summary

Product Size
140 bp
Introns Spanned
2
Targets
1
Tm Difference
0.5°C
Spans 2 introns

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Primers are computationally predicted and not experimentally validated unless indicated by community reviews. For research use only.