Aef1 40 primer pairs found

Drosophila

Show all (including non-specific)

NM_168904 · chr3L

117 1 gene · 4 transcripts

FWD 5' AGGCACTAGCAGCAACAACA 3'

REV 5' TCATGGACTGCTCTTGCTGG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 50%

Product 139 bp

Spans 2 introns

NM_168904 · chr3L

117 1 gene · 4 transcripts

FWD 5' AGGCACTAGCAGCAACAACA 3'

REV 5' GGACTGCTCTTGCTGGGATT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 50%

Product 135 bp

Spans 2 introns

NM_168904 · chr3L

116 1 gene · 3 transcripts

FWD 5' GGAAAGGCACTAGCAGCAAC 3'

REV 5' GCATCATGGACTGCTCTTGC 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 146 bp

Spans 2 introns

NM_168904 · chr3L

116 1 gene · 3 transcripts

FWD 5' TGGAAAGGCACTAGCAGCAA 3'

REV 5' TCATGGACTGCTCTTGCTGG 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 50%

Product 144 bp

Spans 2 introns

NM_168904 · chr3L

116 1 gene · 3 transcripts

FWD 5' GGAAAGGCACTAGCAGCAAC 3'

REV 5' TCATGGACTGCTCTTGCTGG 3'

Fwd Tm 61.1°C

Rev Tm 61.9°C

GC 55%

Product 143 bp

Spans 2 introns

NM_168904 · chr3L

116 1 gene · 3 transcripts

FWD 5' TGGAAAGGCACTAGCAGCAA 3'

REV 5' GGACTGCTCTTGCTGGGATT 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 50%

Product 140 bp

Spans 2 introns

NM_168904 · chr3L

116 1 gene · 3 transcripts

FWD 5' GGAAAGGCACTAGCAGCAAC 3'

REV 5' GGACTGCTCTTGCTGGGATT 3'

Fwd Tm 61.1°C

Rev Tm 62.0°C

GC 55%

Product 139 bp

Spans 2 introns

NM_168904 · chr3L

115 1 gene · 3 transcripts

FWD 5' ATGGAAAGGCACTAGCAGCA 3'

REV 5' TCATGGACTGCTCTTGCTGG 3'

Fwd Tm 61.5°C

Rev Tm 61.9°C

GC 50%

Product 145 bp

Spans 2 introns

NM_168904 · chr3L

115 1 gene · 3 transcripts

FWD 5' ATGGAAAGGCACTAGCAGCA 3'

REV 5' GGACTGCTCTTGCTGGGATT 3'

Fwd Tm 61.5°C

Rev Tm 62.0°C

GC 50%

Product 141 bp

Spans 2 introns

NM_168904 · chr3L

114 1 gene · 3 transcripts

FWD 5' ATGGAAAGGCACTAGCAGCA 3'

REV 5' GCATCATGGACTGCTCTTGC 3'

Fwd Tm 61.5°C

Rev Tm 61.2°C

GC 50%

Product 148 bp

Spans 2 introns

NM_079476 · chr3L

109 Specific

FWD 5' TTAGCTGAAGAAGGGAGCGC 3'

REV 5' TCTTGGGCGCTTCTCTGATG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 143 bp

Spans 1 intron

NM_079476 · chr3L

109 Specific

FWD 5' TTAGCTGAAGAAGGGAGCGC 3'

REV 5' CATCTTGGGCGCTTCTCTGA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 145 bp

Spans 1 intron

NM_079476 · chr3L

109 Specific

FWD 5' TTAGCTGAAGAAGGGAGCGC 3'

REV 5' TCATCTTGGGCGCTTCTCTG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 146 bp

Spans 1 intron

NM_079476 · chr3L

109 Specific

FWD 5' GCGATCTAGAGGCAACGTGT 3'

REV 5' TCTTGGGCGCTTCTCTGATG 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 118 bp

Spans 1 intron

NM_079476 · chr3L

109 Specific

FWD 5' GCGATCTAGAGGCAACGTGT 3'

REV 5' CATCTTGGGCGCTTCTCTGA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 120 bp

Spans 1 intron

NM_079476 · chr3L

109 Specific

FWD 5' GCGATCTAGAGGCAACGTGT 3'

REV 5' TCATCTTGGGCGCTTCTCTG 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 121 bp

Spans 1 intron

NM_079476 · chr3L

109 Specific

FWD 5' TTAGCTGAAGAAGGGAGCGC 3'

REV 5' CTTGGGCGCTTCTCTGATGT 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 142 bp

Spans 1 intron

NM_079476 · chr3L

108 Specific

FWD 5' GCGATCTAGAGGCAACGTGT 3'

REV 5' CTCATCTTGGGCGCTTCTCT 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 122 bp

Spans 1 intron

NM_079476 · chr3L

108 Specific

FWD 5' TTAGCTGAAGAAGGGAGCGC 3'

REV 5' CTCATCTTGGGCGCTTCTCT 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 147 bp

Spans 1 intron

NM_079476 · chr3L

108 Specific

FWD 5' GCGATCTAGAGGCAACGTGT 3'

REV 5' CTTGGGCGCTTCTCTGATGT 3'

Fwd Tm 61.8°C

Rev Tm 62.2°C

GC 55%

Product 117 bp

Spans 1 intron

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