Mis12 50 primer pairs found

Human

Show all (including non-specific)

NM_001258217 · chr17

109 Specific

FWD 5' GTTTAAAGGAGGTGGCGGGA 3'

REV 5' CTCCGTTGCCAATGTCCTCA 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 108 bp

Spans 1 intron

NM_001258217 · chr17

109 Specific

FWD 5' GTTTAAAGGAGGTGGCGGGA 3'

REV 5' ACTCCGTTGCCAATGTCCTC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 109 bp

Spans 1 intron

NM_001258217 · chr17

108 Specific

FWD 5' AATCGTCACCCTCATGGTCG 3'

REV 5' CAGCCTCTGCCACTTCTCTT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001258218 · chr17

108 1 gene · 2 transcripts

FWD 5' AATCGTCACCCTCATGGTCG 3'

REV 5' CCAAAGAACTGGGCCTCGTA 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 106 bp

Spans 1 intron

NM_001258218 · chr17

108 Specific

FWD 5' AATCGTCACCCTCATGGTCG 3'

REV 5' GCGTGAAGCCAAAGAACTGG 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 114 bp

Spans 1 intron

NM_001258218 · chr17

108 Specific

FWD 5' AATCGTCACCCTCATGGTCG 3'

REV 5' GGCGTGAAGCCAAAGAACTG 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 115 bp

Spans 1 intron

NM_001258217 · chr17

108 Specific

FWD 5' AATCGTCACCCTCATGGTCG 3'

REV 5' TTCTCAGCCTCTGCCACTTC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 139 bp

Spans 1 intron

NM_001258217 · chr17

108 Specific

FWD 5' AATCGTCACCCTCATGGTCG 3'

REV 5' TTCTGTTCTCAGCCTCTGCC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 144 bp

Spans 1 intron

NM_001258218 · chr17

108 Specific

FWD 5' TTAAAGGAGGTGGCGGGAAG 3'

REV 5' CCAAAGAACTGGGCCTCGTA 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001258218 · chr17

108 Specific

FWD 5' TTAAAGGAGGTGGCGGGAAG 3'

REV 5' GCGTGAAGCCAAAGAACTGG 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001258218 · chr17

108 Specific

FWD 5' TTAAAGGAGGTGGCGGGAAG 3'

REV 5' GGCGTGAAGCCAAAGAACTG 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001258218 · chr17

108 Specific

FWD 5' AATCGTCACCCTCATGGTCG 3'

REV 5' GTGGCGTGAAGCCAAAGAAC 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 117 bp

Spans 1 intron

NM_001258217 · chr17

108 Specific

FWD 5' AATCGTCACCCTCATGGTCG 3'

REV 5' GTTCTCAGCCTCTGCCACTT 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 140 bp

Spans 1 intron

NM_001258218 · chr17

108 Specific

FWD 5' GTTTAAAGGAGGTGGCGGGA 3'

REV 5' CCAAAGAACTGGGCCTCGTA 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 143 bp

Spans 1 intron

NM_001258217 · chr17

108 Specific

FWD 5' GTTTAAAGGAGGTGGCGGGA 3'

REV 5' TCACTCCGTTGCCAATGTCC 3'

Fwd Tm 61.9°C

Rev Tm 62.4°C

GC 55%

Product 111 bp

Spans 1 intron

NM_001258217 · chr17

108 Specific

FWD 5' TTAAAGGAGGTGGCGGGAAG 3'

REV 5' CTCCGTTGCCAATGTCCTCA 3'

Fwd Tm 61.7°C

Rev Tm 62.1°C

GC 55%

Product 106 bp

Spans 1 intron

NM_001258217 · chr17

108 Specific

FWD 5' TTAAAGGAGGTGGCGGGAAG 3'

REV 5' ACTCCGTTGCCAATGTCCTC 3'

Fwd Tm 61.7°C

Rev Tm 62.1°C

GC 55%

Product 107 bp

Spans 1 intron

NM_001258218 · chr17

108 Specific

FWD 5' GGAAGCCTGTGTGTGCTTCA 3'

REV 5' AAGCCAAAGAACTGGGCCTC 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 55%

Product 129 bp

Spans 1 intron

NM_001258218 · chr17

108 Specific

FWD 5' GGAAGCCTGTGTGTGCTTCA 3'

REV 5' GAAGCCAAAGAACTGGGCCT 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 55%

Product 130 bp

Spans 1 intron

NM_001258217 · chr17

108 Specific

FWD 5' GTTTAAAGGAGGTGGCGGGA 3'

REV 5' CACTCCGTTGCCAATGTCCT 3'

Fwd Tm 61.9°C

Rev Tm 62.4°C

GC 55%

Product 110 bp

Spans 1 intron

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