NKX2-5 30 primer pairs found

Human

Show all (including non-specific)

NM_001166176 · chr5

109 Specific

FWD 5' AGAAAGGGTGTGAACTGCCC 3'

REV 5' CTCACTCTGCCAAGTGCACT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 129 bp

Spans 1 intron

NM_001166176 · chr5

109 Specific

FWD 5' AGAAAGGGTGTGAACTGCCC 3'

REV 5' TCACTCTGCCAAGTGCACTG 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 128 bp

Spans 1 intron

NM_004387 · chr5

108 1 gene · 3 transcripts

FWD 5' CAAGTGTGCGTCTGCCTTTC 3'

REV 5' GTTGTCCGCCTCTGTCTTCT 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 148 bp

Spans 1 intron

NM_001166176 · chr5

108 Specific

FWD 5' AGAAAGGGTGTGAACTGCCC 3'

REV 5' CGACACGTCTCACTCAGCAT 3'

Fwd Tm 62.2°C

Rev Tm 61.7°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001166176 · chr5

107 Specific

FWD 5' AGAAAGGGTGTGAACTGCCC 3'

REV 5' CACCGACACGTCTCACTCAG 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 103 bp

Spans 1 intron

NM_004387 · chr5

107 1 gene · 3 transcripts

FWD 5' CAAGTGTGCGTCTGCCTTTC 3'

REV 5' TTGTCCGCCTCTGTCTTCTC 3'

Fwd Tm 61.5°C

Rev Tm 61.2°C

GC 55%

Product 147 bp

Spans 1 intron

NM_004387 · chr5

106 1 gene · 3 transcripts

FWD 5' GCCTTCTATCCACGTGCCTA 3'

REV 5' TTGTCCGCCTCTGTCTTCTC 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 116 bp

Spans 1 intron

NM_001166175 · chr5

106 Specific

FWD 5' CAAGTGTGCGTCTGCCTTTC 3'

REV 5' AGGGAGACAGACGGTGACTT 3'

Fwd Tm 61.5°C

Rev Tm 62.2°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001166176 · chr5

106 1 gene · 2 transcripts

FWD 5' TATCCACGTGCCTACAGCGA 3'

REV 5' CTCAAGGCGCTGGAGAACAA 3'

Fwd Tm 62.8°C

Rev Tm 62.3°C

GC 55%

Product 113 bp

Spans 1 intron

NM_001166175 · chr5

106 1 gene · 2 transcripts

FWD 5' TATCCACGTGCCTACAGCGA 3'

REV 5' CTCAAGGCGCTGGAGAACAA 3'

Fwd Tm 62.8°C

Rev Tm 62.3°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001166176 · chr5

106 1 gene · 2 transcripts

FWD 5' CTATCCACGTGCCTACAGCG 3'

REV 5' CTCAAGGCGCTGGAGAACAA 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 60%

Product 114 bp

Spans 1 intron

NM_001166175 · chr5

106 Specific

FWD 5' CTATCCACGTGCCTACAGCG 3'

REV 5' AGGGAGACAGACGGTGACTT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 60%

Product 90 bp

Spans 1 intron

NM_001166176 · chr5

106 1 gene · 2 transcripts

FWD 5' CTATCCACGTGCCTACAGCG 3'

REV 5' AAAGTCAGGCTGGCTCAAGG 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 60%

Product 127 bp

Spans 1 intron

NM_001166176 · chr5

106 1 gene · 2 transcripts

FWD 5' TATCCACGTGCCTACAGCGA 3'

REV 5' AAAGTCAGGCTGGCTCAAGG 3'

Fwd Tm 62.8°C

Rev Tm 62.2°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001166176 · chr5

106 1 gene · 2 transcripts

FWD 5' AAGTGTGCGTCTGCCTTTCC 3'

REV 5' CTCAAGGCGCTGGAGAACAA 3'

Fwd Tm 62.8°C

Rev Tm 62.3°C

GC 55%

Product 149 bp

Spans 1 intron

NM_004387 · chr5

106 1 gene · 3 transcripts

FWD 5' GCCTTCTATCCACGTGCCTA 3'

REV 5' GTTGTCCGCCTCTGTCTTCT 3'

Fwd Tm 61.1°C

Rev Tm 61.5°C

GC 55%

Product 117 bp

Spans 1 intron

NM_004387 · chr5

106 1 gene · 3 transcripts

FWD 5' TCTATCCACGTGCCTACAGC 3'

REV 5' TTGTCCGCCTCTGTCTTCTC 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 112 bp

Spans 1 intron

NM_001166175 · chr5

106 Specific

FWD 5' AAGTGTGCGTCTGCCTTTCC 3'

REV 5' AGGGAGACAGACGGTGACTT 3'

Fwd Tm 62.8°C

Rev Tm 62.2°C

GC 55%

Product 125 bp

Spans 1 intron

NM_001166176 · chr5

106 1 gene · 2 transcripts

FWD 5' CAAGTGTGCGTCTGCCTTTC 3'

REV 5' CTCAAGGCGCTGGAGAACAA 3'

Fwd Tm 61.5°C

Rev Tm 62.3°C

GC 55%

Product 150 bp

Spans 1 intron

NM_004387 · chr5

106 1 gene · 3 transcripts

FWD 5' TCTATCCACGTGCCTACAGC 3'

REV 5' GTTGTCCGCCTCTGTCTTCT 3'

Fwd Tm 61.1°C

Rev Tm 61.5°C

GC 55%

Product 113 bp

Spans 1 intron

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