Nck1 30 primer pairs found

Human

Show all (including non-specific)

NM_006153 · chr3

109 Specific

FWD 5' CTCAGTGCTGAAGCTGCTGA 3'

REV 5' ACTCGCCACCAGGACTTAGA 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001291999 · chr3

109 Specific

FWD 5' GGAAGTGCTGAAGCTGCTGA 3'

REV 5' ACTCGCCACCAGGACTTAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 145 bp

Spans 1 intron

NM_006153 · chr3

106 Specific

FWD 5' CTCAGTGCTGAAGCTGCTGA 3'

REV 5' AACTCGCCACCAGGACTTAG 3'

Fwd Tm 62.0°C

Rev Tm 61.3°C

GC 55%

Product 146 bp

Spans 1 intron

NM_006153 · chr3

106 Specific

FWD 5' CTCAGTGCTGAAGCTGCTGA 3'

REV 5' GAACTCGCCACCAGGACTTA 3'

Fwd Tm 62.0°C

Rev Tm 61.3°C

GC 55%

Product 147 bp

Spans 1 intron

NM_006153 · chr3

106 Specific

FWD 5' CCTCCTCAGTGCTGAAGCTG 3'

REV 5' ACTCGCCACCAGGACTTAGA 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 60%

Product 149 bp

Spans 1 intron

NM_006153 · chr3

105 Specific

FWD 5' CTCAGTGCTGAAGCTGCTGA 3'

REV 5' CTCGCCACCAGGACTTAGAA 3'

Fwd Tm 62.0°C

Rev Tm 61.0°C

GC 55%

Product 144 bp

Spans 1 intron

NM_001291999 · chr3

105 Specific

FWD 5' GGAAGTGCTGAAGCTGCTGA 3'

REV 5' AACTCGCCACCAGGACTTAG 3'

Fwd Tm 62.4°C

Rev Tm 61.3°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001291999 · chr3

105 Specific

FWD 5' GGAAGTGCTGAAGCTGCTGA 3'

REV 5' GAACTCGCCACCAGGACTTA 3'

Fwd Tm 62.4°C

Rev Tm 61.3°C

GC 55%

Product 147 bp

Spans 1 intron

NM_006153 · chr3

104 Specific

FWD 5' TCAGTGCTGAAGCTGCTGAA 3'

REV 5' AACTCGCCACCAGGACTTAG 3'

Fwd Tm 61.5°C

Rev Tm 61.3°C

GC 50%

Product 145 bp

Spans 1 intron

NM_006153 · chr3

104 Specific

FWD 5' TCAGTGCTGAAGCTGCTGAA 3'

REV 5' GAACTCGCCACCAGGACTTA 3'

Fwd Tm 61.5°C

Rev Tm 61.3°C

GC 50%

Product 146 bp

Spans 1 intron

NM_006153 · chr3

104 Specific

FWD 5' TCAGTGCTGAAGCTGCTGAA 3'

REV 5' ACTCGCCACCAGGACTTAGA 3'

Fwd Tm 61.5°C

Rev Tm 62.3°C

GC 50%

Product 144 bp

Spans 1 intron

NM_006153 · chr3

104 Specific

FWD 5' CTCCTCAGTGCTGAAGCTGC 3'

REV 5' ACTCGCCACCAGGACTTAGA 3'

Fwd Tm 62.9°C

Rev Tm 62.3°C

GC 60%

Product 148 bp

Spans 1 intron

NM_001291999 · chr3

103 Specific

FWD 5' GGAAGTGCTGAAGCTGCTGA 3'

REV 5' CTCGCCACCAGGACTTAGAA 3'

Fwd Tm 62.4°C

Rev Tm 61.0°C

GC 55%

Product 144 bp

Spans 1 intron

NM_006153 · chr3

103 Specific

FWD 5' TCCTCAGTGCTGAAGCTGCT 3'

REV 5' ACTCGCCACCAGGACTTAGA 3'

Fwd Tm 63.4°C

Rev Tm 62.3°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001291999 · chr3

101 1 gene · 2 transcripts

FWD 5' AACAGTGCTCGGAAAGCATC 3'

REV 5' GGAGATGCAGAATCTGGCAC 3'

Fwd Tm 60.3°C

Rev Tm 60.5°C

GC 50%

Product 98 bp

Spans 1 intron

NM_001291999 · chr3

101 Specific

FWD 5' GGGAAGTGCTGAAGCTGCTG 3'

REV 5' ACTCGCCACCAGGACTTAGA 3'

Fwd Tm 63.4°C

Rev Tm 62.3°C

GC 60%

Product 146 bp

Spans 1 intron

NM_001291999 · chr3

100 1 gene · 2 transcripts

FWD 5' ACAGTGCTCGGAAAGCATCT 3'

REV 5' GGAGATGCAGAATCTGGCAC 3'

Fwd Tm 61.3°C

Rev Tm 60.5°C

GC 50%

Product 97 bp

Spans 1 intron

NM_001291999 · chr3

100 1 gene · 2 transcripts

FWD 5' AACAGTGCTCGGAAAGCATC 3'

REV 5' CAGGAGATGCAGAATCTGGC 3'

Fwd Tm 60.3°C

Rev Tm 60.2°C

GC 50%

Product 100 bp

Spans 1 intron

NM_001291999 · chr3

100 1 gene · 2 transcripts

FWD 5' AACAGTGCTCGGAAAGCATC 3'

REV 5' GCAGGAGATGCAGAATCTGG 3'

Fwd Tm 60.3°C

Rev Tm 60.2°C

GC 50%

Product 101 bp

Spans 1 intron

NM_001291999 · chr3

100 1 gene · 2 transcripts

FWD 5' AGTGCTGAAGCTGCTGAAAG 3'

REV 5' CTCGCCACCAGGACTTAGAA 3'

Fwd Tm 60.3°C

Rev Tm 61.0°C

GC 50%

Product 141 bp

Spans 1 intron

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