PRKAA1 80 primer pairs found

Human

Show all (including non-specific)

NM_001355036 · chr5

118 Specific

FWD 5' GCCTTGATGTGGTACCAGGT 3'

REV 5' GGAACAGACGCCGACTTTCT 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001355036 · chr5

117 Specific

FWD 5' GCCTTGATGTGGTACCAGGT 3'

REV 5' TGGAACAGACGCCGACTTTC 3'

Fwd Tm 61.7°C

Rev Tm 62.2°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001355036 · chr5

117 Specific

FWD 5' AGCCTTGATGTGGTACCAGG 3'

REV 5' GGAACAGACGCCGACTTTCT 3'

Fwd Tm 61.4°C

Rev Tm 62.0°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001355036 · chr5

117 Specific

FWD 5' GGAGCCTTGATGTGGTACCA 3'

REV 5' GGAACAGACGCCGACTTTCT 3'

Fwd Tm 61.4°C

Rev Tm 62.0°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001355036 · chr5

117 Specific

FWD 5' TCGGAGCCTTGATGTGGTAC 3'

REV 5' GGAACAGACGCCGACTTTCT 3'

Fwd Tm 61.3°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001355036 · chr5

116 Specific

FWD 5' GCCTTGATGTGGTACCAGGT 3'

REV 5' TTGGAACAGACGCCGACTTT 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001355036 · chr5

116 Specific

FWD 5' AGCCTTGATGTGGTACCAGG 3'

REV 5' TGGAACAGACGCCGACTTTC 3'

Fwd Tm 61.4°C

Rev Tm 62.2°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001355036 · chr5

116 Specific

FWD 5' GCCTTGATGTGGTACCAGGT 3'

REV 5' GTTGGAACAGACGCCGACTT 3'

Fwd Tm 61.7°C

Rev Tm 62.5°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001355036 · chr5

116 Specific

FWD 5' GGAGCCTTGATGTGGTACCA 3'

REV 5' TGGAACAGACGCCGACTTTC 3'

Fwd Tm 61.4°C

Rev Tm 62.2°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001355036 · chr5

116 Specific

FWD 5' CGGAGCCTTGATGTGGTACC 3'

REV 5' TGGAACAGACGCCGACTTTC 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 60%

Product 141 bp

Spans 2 introns

NM_001355029 · chr5

110 1 gene · 7 transcripts

FWD 5' TCCTGCTTGATGCACACATG 3'

REV 5' CCTCTGGGCCTGCATACAAT 3'

Fwd Tm 60.4°C

Rev Tm 61.9°C

GC 50%

Product 150 bp

Spans 2 introns

NM_001355029 · chr5

110 1 gene · 7 transcripts

FWD 5' CCTGCTTGATGCACACATGA 3'

REV 5' CCTCTGGGCCTGCATACAAT 3'

Fwd Tm 60.4°C

Rev Tm 61.9°C

GC 50%

Product 149 bp

Spans 2 introns

NM_006251 · chr5

110 1 gene · 7 transcripts

FWD 5' TCCTGCTTGATGCACACATG 3'

REV 5' CCTCTGGGCCTGCATACAAT 3'

Fwd Tm 60.4°C

Rev Tm 61.9°C

GC 50%

Product 150 bp

Spans 2 introns

NM_006251 · chr5

110 1 gene · 7 transcripts

FWD 5' CCTGCTTGATGCACACATGA 3'

REV 5' CCTCTGGGCCTGCATACAAT 3'

Fwd Tm 60.4°C

Rev Tm 61.9°C

GC 50%

Product 149 bp

Spans 2 introns

NM_001355028 · chr5

110 1 gene · 7 transcripts

FWD 5' TCCTGCTTGATGCACACATG 3'

REV 5' CCTCTGGGCCTGCATACAAT 3'

Fwd Tm 60.4°C

Rev Tm 61.9°C

GC 50%

Product 150 bp

Spans 2 introns

NM_001355028 · chr5

110 1 gene · 7 transcripts

FWD 5' CCTGCTTGATGCACACATGA 3'

REV 5' CCTCTGGGCCTGCATACAAT 3'

Fwd Tm 60.4°C

Rev Tm 61.9°C

GC 50%

Product 149 bp

Spans 2 introns

NM_206907 · chr5

110 1 gene · 7 transcripts

FWD 5' TCCTGCTTGATGCACACATG 3'

REV 5' CCTCTGGGCCTGCATACAAT 3'

Fwd Tm 60.4°C

Rev Tm 61.9°C

GC 50%

Product 150 bp

Spans 2 introns

NM_206907 · chr5

110 1 gene · 7 transcripts

FWD 5' CCTGCTTGATGCACACATGA 3'

REV 5' CCTCTGGGCCTGCATACAAT 3'

Fwd Tm 60.4°C

Rev Tm 61.9°C

GC 50%

Product 149 bp

Spans 2 introns

NM_001355037 · chr5

109 1 gene · 2 transcripts

FWD 5' AAACAGGCTCCACGAAGGAG 3'

REV 5' CCTCTGGGCCTGCATACAAT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 185 bp

Spans 2 introns

NM_001355037 · chr5

109 1 gene · 2 transcripts

FWD 5' AAACAGGCTCCACGAAGGAG 3'

REV 5' TCTACCTCTGGGCCTGCATA 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 189 bp

Spans 2 introns

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