AMIGO2 30 primer pairs found

Human

Show all (including non-specific)

NM_181847 · chr12

108 1 gene · 3 transcripts

FWD 5' GCAAGAGTGACAGACACGGA 3'

REV 5' TCTGAGTCTCTTCCCGGTGT 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001143668 · chr12

108 1 gene · 2 transcripts

FWD 5' GCACCGACTAACCCAGGAAA 3'

REV 5' CTCAGCCTCCCACCAGTGAA 3'

Fwd Tm 61.8°C

Rev Tm 63.7°C

GC 55%

Product 128 bp

Spans 2 introns

NM_001370299 · chr12

108 1 gene · 2 transcripts

FWD 5' GCACCGACTAACCCAGGAAA 3'

REV 5' CTCAGCCTCCCACCAGTGAA 3'

Fwd Tm 61.8°C

Rev Tm 63.7°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001370299 · chr12

108 1 gene · 2 transcripts

FWD 5' CAGCACCGACTAACCCAGGA 3'

REV 5' CTCAGCCTCCCACCAGTGAA 3'

Fwd Tm 63.6°C

Rev Tm 63.7°C

GC 60%

Product 135 bp

Spans 2 introns

NM_181847 · chr12

106 1 gene · 3 transcripts

FWD 5' GTGACAGACACGGACAGACG 3'

REV 5' TGGTCGCCTCTGAGTCTCTT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 60%

Product 148 bp

Spans 1 intron

NM_181847 · chr12

106 1 gene · 3 transcripts

FWD 5' GTGACAGACACGGACAGACG 3'

REV 5' ATGGTCGCCTCTGAGTCTCT 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 60%

Product 149 bp

Spans 1 intron

NM_181847 · chr12

106 1 gene · 3 transcripts

FWD 5' GTGACAGACACGGACAGACG 3'

REV 5' TGAGTCTCTTCCCGGTGTCT 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 60%

Product 138 bp

Spans 1 intron

NM_181847 · chr12

106 1 gene · 3 transcripts

FWD 5' GTGACAGACACGGACAGACG 3'

REV 5' TCTGAGTCTCTTCCCGGTGT 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 60%

Product 140 bp

Spans 1 intron

NM_181847 · chr12

105 1 gene · 3 transcripts

FWD 5' AGTGACAGACACGGACAGAC 3'

REV 5' TCTGAGTCTCTTCCCGGTGT 3'

Fwd Tm 61.1°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 1 intron

NM_181847 · chr12

105 1 gene · 3 transcripts

FWD 5' GCAAGAGTGACAGACACGGA 3'

REV 5' CTGAGTCTCTTCCCGGTGTC 3'

Fwd Tm 61.6°C

Rev Tm 61.4°C

GC 55%

Product 145 bp

Spans 1 intron

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