Act5C 50 primer pairs found

Drosophila

Show all (including non-specific)

NM_167053 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' TCGACAACCAGAGCAGCAAC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 146 bp

Spans 1 intron

NM_167053 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' GTCGACAACCAGAGCAGCAA 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 147 bp

Spans 1 intron

NM_167053 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' TTGTCGACAACCAGAGCAGC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001297986 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' TCGACAACCAGAGCAGCAAC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001297986 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' GTCGACAACCAGAGCAGCAA 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001297986 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' TTGTCGACAACCAGAGCAGC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001014726 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' TCGACAACCAGAGCAGCAAC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001014726 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' GTCGACAACCAGAGCAGCAA 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001014726 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' TTGTCGACAACCAGAGCAGC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001014725 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' TCGACAACCAGAGCAGCAAC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001014725 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' GTCGACAACCAGAGCAGCAA 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001014725 · chrX

107 1 gene · 4 transcripts

FWD 5' ACACAAAGCCGCTCCATCAG 3'

REV 5' TTGTCGACAACCAGAGCAGC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 149 bp

Spans 1 intron

NM_078497 · chrX

107 Specific

FWD 5' TCGCCACTTGCGTTTACAGT 3'

REV 5' CAAATCCGGCCTTGCACATG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 50%

Product 133 bp

Spans 1 intron

NM_078497 · chrX

107 Specific

FWD 5' TCCTTTCAAACCGTGCGGTC 3'

REV 5' CGACAACCAGAGCAGCAACT 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 55%

Product 133 bp

Spans 1 intron

NM_078497 · chrX

107 Specific

FWD 5' CCTCGCCACTTGCGTTTACA 3'

REV 5' CGACAACCAGAGCAGCAACT 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 55%

Product 102 bp

Spans 1 intron

NM_078497 · chrX

107 Specific

FWD 5' TCCTTTCAAACCGTGCGGTC 3'

REV 5' TCGACAACCAGAGCAGCAAC 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 55%

Product 134 bp

Spans 1 intron

NM_078497 · chrX

107 Specific

FWD 5' TCCTTTCAAACCGTGCGGTC 3'

REV 5' GTCGACAACCAGAGCAGCAA 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 55%

Product 135 bp

Spans 1 intron

NM_078497 · chrX

107 Specific

FWD 5' TCCTTTCAAACCGTGCGGTC 3'

REV 5' TTGTCGACAACCAGAGCAGC 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 55%

Product 137 bp

Spans 1 intron

NM_078497 · chrX

107 Specific

FWD 5' CCTCGCCACTTGCGTTTACA 3'

REV 5' TCGACAACCAGAGCAGCAAC 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 55%

Product 103 bp

Spans 1 intron

NM_078497 · chrX

107 Specific

FWD 5' CCTCGCCACTTGCGTTTACA 3'

REV 5' GTCGACAACCAGAGCAGCAA 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 55%

Product 104 bp

Spans 1 intron

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