Alp12 50 primer pairs found

Drosophila

Show all (including non-specific)

NM_001201920 · chr2L

103 Specific

FWD 5' TATTGTCGCCCGATTGGAGC 3'

REV 5' CGTCTGGATCGGCCTAATCT 3'

Fwd Tm 62.2°C

Rev Tm 60.9°C

GC 55%

Product 125 bp

Spans 1 intron

NM_136119 · chr2L

103 Specific

FWD 5' GTGAGCGGCATTCAAATCGT 3'

REV 5' CGTCTGGATCGGCCTAATCT 3'

Fwd Tm 60.9°C

Rev Tm 60.9°C

GC 50%

Product 116 bp

Spans 1 intron

NM_136119 · chr2L

103 Specific

FWD 5' CACTCACAGCTGTTCCCATG 3'

REV 5' GTCATGTCCACATCGCTGAG 3'

Fwd Tm 60.6°C

Rev Tm 60.3°C

GC 55%

Product 132 bp

Spans 1 intron

NM_136119 · chr2L

103 Specific

FWD 5' AATTCTCGTCAGTGAGCGGC 3'

REV 5' CGTCTGGATCGGCCTAATCT 3'

Fwd Tm 62.3°C

Rev Tm 60.9°C

GC 55%

Product 127 bp

Spans 1 intron

NM_136119 · chr2L

103 Specific

FWD 5' CACTCACAGCTGTTCCCATG 3'

REV 5' CTTCCGTCATGTCCACATCG 3'

Fwd Tm 60.6°C

Rev Tm 60.3°C

GC 55%

Product 137 bp

Spans 1 intron

NM_136119 · chr2L

103 Specific

FWD 5' GTGAGCGGCATTCAAATCGT 3'

REV 5' ACGTCTGGATCGGCCTAATC 3'

Fwd Tm 60.9°C

Rev Tm 61.1°C

GC 50%

Product 117 bp

Spans 1 intron

NM_136119 · chr2L

103 Specific

FWD 5' GGAATTCTCGTCAGTGAGCG 3'

REV 5' CATTAACGTCTGGATCGGCC 3'

Fwd Tm 60.2°C

Rev Tm 60.4°C

GC 55%

Product 135 bp

Spans 1 intron

NM_136119 · chr2L

103 Specific

FWD 5' GAATTCTCGTCAGTGAGCGG 3'

REV 5' CATTAACGTCTGGATCGGCC 3'

Fwd Tm 60.2°C

Rev Tm 60.4°C

GC 55%

Product 134 bp

Spans 1 intron

NM_136119 · chr2L

103 Specific

FWD 5' CACTCACAGCTGTTCCCATG 3'

REV 5' CGTCATGTCCACATCGCTGA 3'

Fwd Tm 60.6°C

Rev Tm 62.0°C

GC 55%

Product 133 bp

Spans 1 intron

NM_136119 · chr2L

103 Specific

FWD 5' CACTCACAGCTGTTCCCATG 3'

REV 5' CTGCACTTCCGTCATGTCCA 3'

Fwd Tm 60.6°C

Rev Tm 62.0°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001273647 · chr2L

103 Specific

FWD 5' CGCACTCACTAATGGCAGCA 3'

REV 5' ACGTCTGGATCGGCCTAATC 3'

Fwd Tm 62.6°C

Rev Tm 61.1°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001273647 · chr2L

101 Specific

FWD 5' CGCACTCACTAATGGCAGCA 3'

REV 5' CGTCTGGATCGGCCTAATCT 3'

Fwd Tm 62.6°C

Rev Tm 60.9°C

GC 55%

Product 125 bp

Spans 1 intron

NM_001273647 · chr2L

101 Specific

FWD 5' GGAGATGAAGCTGAAGCCTC 3'

REV 5' CTTCCGTCATGTCCACATCG 3'

Fwd Tm 59.9°C

Rev Tm 60.3°C

GC 55%

Product 140 bp

Spans 1 intron

NM_001273647 · chr2L

101 Specific

FWD 5' GCACTCACTAATGGCAGCAA 3'

REV 5' CATTAACGTCTGGATCGGCC 3'

Fwd Tm 60.4°C

Rev Tm 60.4°C

GC 50%

Product 130 bp

Spans 1 intron

NM_001273647 · chr2L

101 Specific

FWD 5' GGAGATGAAGCTGAAGCCTC 3'

REV 5' GTCATGTCCACATCGCTGAG 3'

Fwd Tm 59.9°C

Rev Tm 60.3°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001273647 · chr2L

101 Specific

FWD 5' GCACTCACTAATGGCAGCAA 3'

REV 5' CGTCTGGATCGGCCTAATCT 3'

Fwd Tm 60.4°C

Rev Tm 60.9°C

GC 50%

Product 124 bp

Spans 1 intron

NM_001273647 · chr2L

101 Specific

FWD 5' TGGAGATGAAGCTGAAGCCT 3'

REV 5' CGTCATGTCCACATCGCTGA 3'

Fwd Tm 60.6°C

Rev Tm 62.0°C

GC 50%

Product 137 bp

Spans 1 intron

NM_001273647 · chr2L

100 Specific

FWD 5' TGGAGATGAAGCTGAAGCCT 3'

REV 5' GTCATGTCCACATCGCTGAG 3'

Fwd Tm 60.6°C

Rev Tm 60.3°C

GC 50%

Product 136 bp

Spans 1 intron

NM_001273647 · chr2L

100 Specific

FWD 5' TGGAGATGAAGCTGAAGCCT 3'

REV 5' CTGCACTTCCGTCATGTCCA 3'

Fwd Tm 60.6°C

Rev Tm 62.0°C

GC 50%

Product 146 bp

Spans 1 intron

NM_001273647 · chr2L

100 Specific

FWD 5' GCACTCACTAATGGCAGCAA 3'

REV 5' ACGTCTGGATCGGCCTAATC 3'

Fwd Tm 60.4°C

Rev Tm 61.1°C

GC 50%

Product 125 bp

Spans 1 intron

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