Ama 30 primer pairs found

Drosophila

Show all (including non-specific)

NM_169160 · chr3R

99 Specific

FWD 5' AAAGACCAGACATGGCGCGT 3'

REV 5' ACGTCCTTGCTGATCTGGCT 3'

Fwd Tm 64.4°C

Rev Tm 63.5°C

GC 55%

Product 112 bp

Spans 1 intron

NM_169160 · chr3R

97 Specific

FWD 5' AAAGACCAGACATGGCGCGT 3'

REV 5' AACTCCACAGAGTCGCCCAC 3'

Fwd Tm 64.4°C

Rev Tm 64.1°C

GC 55%

Product 142 bp

Spans 1 intron

NM_169160 · chr3R

97 Specific

FWD 5' AAAGACCAGACATGGCGCGT 3'

REV 5' GAACTCCACAGAGTCGCCCA 3'

Fwd Tm 64.4°C

Rev Tm 63.8°C

GC 55%

Product 143 bp

Spans 1 intron

NM_169160 · chr3R

97 Specific

FWD 5' AAAGACCAGACATGGCGCGT 3'

REV 5' TGAACTCCACAGAGTCGCCC 3'

Fwd Tm 64.4°C

Rev Tm 63.8°C

GC 55%

Product 144 bp

Spans 1 intron

NM_169160 · chr3R

97 Specific

FWD 5' AAAGACCAGACATGGCGCGT 3'

REV 5' ACCACGTCCTTGCTGATCTG 3'

Fwd Tm 64.4°C

Rev Tm 61.8°C

GC 55%

Product 115 bp

Spans 1 intron

NM_169160 · chr3R

97 Specific

FWD 5' AAAGACCAGACATGGCGCGT 3'

REV 5' CACCACGTCCTTGCTGATCT 3'

Fwd Tm 64.4°C

Rev Tm 61.8°C

GC 55%

Product 116 bp

Spans 1 intron

NM_169160 · chr3R

97 Specific

FWD 5' AAAGACCAGACATGGCGCGT 3'

REV 5' CACGTCCTTGCTGATCTGGC 3'

Fwd Tm 64.4°C

Rev Tm 63.1°C

GC 55%

Product 113 bp

Spans 1 intron

NM_169160 · chr3R

97 Specific

FWD 5' AAAGACCAGACATGGCGCGT 3'

REV 5' TTGAACTCCACAGAGTCGCC 3'

Fwd Tm 64.4°C

Rev Tm 61.7°C

GC 55%

Product 145 bp

Spans 1 intron

NM_169160 · chr3R

96 Specific

FWD 5' AAAGACCAGACATGGCGCGT 3'

REV 5' CGTCCTTGCTGATCTGGCTG 3'

Fwd Tm 64.4°C

Rev Tm 62.9°C

GC 55%

Product 111 bp

Spans 1 intron

NM_169160 · chr3R

96 Specific

FWD 5' AAAGACCAGACATGGCGCGT 3'

REV 5' CCACCACGTCCTTGCTGATC 3'

Fwd Tm 64.4°C

Rev Tm 62.9°C

GC 55%

Product 117 bp

Spans 1 intron

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