Arf51F 50 primer pairs found

Drosophila

Show all (including non-specific)

NM_166089 · chr2R

116 Specific

FWD 5' GACTTGGCAACGAGCTGATG 3'

REV 5' GTCCAGTCCGAGCATGAGAA 3'

Fwd Tm 61.1°C

Rev Tm 61.3°C

GC 55%

Product 126 bp

Spans 2 introns

NM_166089 · chr2R

116 Specific

FWD 5' CTTGGCAACGAGCTGATGAC 3'

REV 5' GTCCAGTCCGAGCATGAGAA 3'

Fwd Tm 61.1°C

Rev Tm 61.3°C

GC 55%

Product 124 bp

Spans 2 introns

NM_166089 · chr2R

116 1 gene · 4 transcripts

FWD 5' TGGAACGAATTGGGCTGGAA 3'

REV 5' TCCCATGGTGCTGATGTGTC 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 50%

Product 112 bp

Spans 2 introns

NM_166090 · chr2R

116 1 gene · 4 transcripts

FWD 5' TAACGGATTTCAGGCTGGCG 3'

REV 5' TCGCATTTCCTTGTTGCCGA 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 142 bp

Spans 2 introns

NM_166090 · chr2R

116 1 gene · 4 transcripts

FWD 5' GTGGAACGAATTGGGCTGGA 3'

REV 5' TCGCATTTCCTTGTTGCCGA 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 123 bp

Spans 2 introns

NM_166091 · chr2R

115 Specific

FWD 5' GCGATAAGCCCGCAATTGAG 3'

REV 5' TTCCCATGGTGCTGATGTGT 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 144 bp

Spans 2 introns

NM_166090 · chr2R

115 1 gene · 4 transcripts

FWD 5' ATTTCAGGCTGGCGTGGAAC 3'

REV 5' CCCATGGTGCTGATGTGTCA 3'

Fwd Tm 63.0°C

Rev Tm 62.2°C

GC 55%

Product 96 bp

Spans 2 introns

NM_166091 · chr2R

115 Specific

FWD 5' CAGCGATAAGCCCGCAATTG 3'

REV 5' TTCCCATGGTGCTGATGTGT 3'

Fwd Tm 61.8°C

Rev Tm 61.5°C

GC 55%

Product 146 bp

Spans 2 introns

NM_166091 · chr2R

115 Specific

FWD 5' CCAGCGATAAGCCCGCAATT 3'

REV 5' CCCATGGTGCTGATGTGTCA 3'

Fwd Tm 63.1°C

Rev Tm 62.2°C

GC 55%

Product 145 bp

Spans 2 introns

NM_166091 · chr2R

114 Specific

FWD 5' CCAGCGATAAGCCCGCAATT 3'

REV 5' TCCCATGGTGCTGATGTGTC 3'

Fwd Tm 63.1°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 2 introns

NM_166088 · chr2R

109 1 gene · 4 transcripts

FWD 5' GCGCACTTTGGCACTACATG 3'

REV 5' TCCCATGGTGCTGATGTGTC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 106 bp

Spans 1 intron

NM_079027 · chr2R

109 1 gene · 4 transcripts

FWD 5' GCGCACTTTGGCACTACATG 3'

REV 5' TCCCATGGTGCTGATGTGTC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 1 intron

NM_166088 · chr2R

108 1 gene · 4 transcripts

FWD 5' GCGCACTTTGGCACTACATG 3'

REV 5' CCCATGGTGCTGATGTGTCA 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 105 bp

Spans 1 intron

NM_079027 · chr2R

108 1 gene · 4 transcripts

FWD 5' GCGCACTTTGGCACTACATG 3'

REV 5' CCCATGGTGCTGATGTGTCA 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 134 bp

Spans 1 intron

NM_079027 · chr2R

108 Specific

FWD 5' GTCGGAGACTTGGATTGGCT 3'

REV 5' CCCACAGTGGGTATCGTTGT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 185 bp

Spans 2 introns

NM_079027 · chr2R

108 1 gene · 4 transcripts

FWD 5' TAACGGATTTCAGGCTGGCG 3'

REV 5' CCCATGGTGCTGATGTGTCA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 113 bp

Spans 1 intron

NM_079027 · chr2R

108 Specific

FWD 5' GTCGGAGACTTGGATTGGCT 3'

REV 5' AAAGCCCACAGTGGGTATCG 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 189 bp

Spans 2 introns

NM_079027 · chr2R

108 1 gene · 4 transcripts

FWD 5' GTGGAACGAATTGGGCTGGA 3'

REV 5' CCCATGGTGCTGATGTGTCA 3'

Fwd Tm 62.5°C

Rev Tm 62.2°C

GC 55%

Product 94 bp

Spans 1 intron

NM_079027 · chr2R

108 1 gene · 4 transcripts

FWD 5' TAACGGATTTCAGGCTGGCG 3'

REV 5' TCCCATGGTGCTGATGTGTC 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 114 bp

Spans 1 intron

NM_166088 · chr2R

108 1 gene · 5 transcripts

FWD 5' GCAGGATCTGCCTGATGCAA 3'

REV 5' CGGATGTGGCACATGATGGT 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 55%

Product 107 bp

Spans 1 intron

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