NM_001282674 · chrX

94 1 gene · 2 transcripts

FWD 5' CTTCGGTGCAGTCGTCACTC 3'

REV 5' GGCTGCTCCATCTCTTCGTT 3'

Fwd Tm 62.6°C

Rev Tm 61.9°C

GC 60%

Product 162 bp

Spans 1 intron

NCBI BLAST

NM_001282674 · chrX

93 1 gene · 2 transcripts

FWD 5' CTTCGGTGCAGTCGTCACTC 3'

REV 5' AGGCTGCTCCATCTCTTCGT 3'

Fwd Tm 62.6°C

Rev Tm 62.9°C

GC 60%

Product 163 bp

Spans 1 intron

NCBI BLAST

NM_001282674 · chrX

92 1 gene · 2 transcripts

FWD 5' GTCACTCGCGTCTGGCTACC 3'

REV 5' AGGCTGCTCCATCTCTTCGT 3'

Fwd Tm 64.7°C

Rev Tm 62.9°C

GC 65%

Product 150 bp

Spans 1 intron

NCBI BLAST

NM_001282674 · chrX

92 1 gene · 2 transcripts

FWD 5' CACTCGCGTCTGGCTACCAG 3'

REV 5' AGGCTGCTCCATCTCTTCGT 3'

Fwd Tm 64.7°C

Rev Tm 62.9°C

GC 65%

Product 148 bp

Spans 1 intron

NCBI BLAST

NM_001282674 · chrX

92 1 gene · 2 transcripts

FWD 5' CTTCGGTGCAGTCGTCACTC 3'

REV 5' CAAAGGGCGGTCTTCCTCTC 3'

Fwd Tm 62.6°C

Rev Tm 62.4°C

GC 60%

Product 193 bp

Spans 1 intron

NCBI BLAST

NM_001282674 · chrX

92 1 gene · 2 transcripts

FWD 5' CTTCGGTGCAGTCGTCACTC 3'

REV 5' CTGCATAGGCTGCTCCATCT 3'

Fwd Tm 62.6°C

Rev Tm 61.5°C

GC 60%

Product 169 bp

Spans 1 intron

NCBI BLAST

NM_001282674 · chrX

92 1 gene · 2 transcripts

FWD 5' CTTCGGTGCAGTCGTCACTC 3'

REV 5' GGCGGTCTTCCTCTCCATTC 3'

Fwd Tm 62.6°C

Rev Tm 62.2°C

GC 60%

Product 188 bp

Spans 1 intron

NCBI BLAST

NM_001282674 · chrX

92 1 gene · 2 transcripts

FWD 5' CTTCGGTGCAGTCGTCACTC 3'

REV 5' TGCATAGGCTGCTCCATCTC 3'

Fwd Tm 62.6°C

Rev Tm 61.5°C

GC 60%

Product 168 bp

Spans 1 intron

NCBI BLAST

NM_001282674 · chrX

92 1 gene · 2 transcripts

FWD 5' CTTCGGTGCAGTCGTCACTC 3'

REV 5' TCTGCATAGGCTGCTCCATC 3'

Fwd Tm 62.6°C

Rev Tm 61.5°C

GC 60%

Product 170 bp

Spans 1 intron

NCBI BLAST

NM_001282674 · chrX

91 1 gene · 2 transcripts

FWD 5' GTCACTCGCGTCTGGCTACC 3'

REV 5' GGCTGCTCCATCTCTTCGTT 3'

Fwd Tm 64.7°C

Rev Tm 61.9°C

GC 65%

Product 149 bp

Spans 1 intron

NCBI BLAST

NM_014380 · chrX

80 Single Exon Specific

FWD 5' AATCCTGCTGGTCAGAGCAC 3'

REV 5' GGCTGCTCCATCTCTTCGTT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 137 bp

Single-exon gene (no intron)

NCBI BLAST

NM_014380 · chrX

79 Single Exon 1 gene · 4 transcripts

FWD 5' AACGAAGAGATGGAGCAGCC 3'

REV 5' GCCTATTGGGTATGGCCCAT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 142 bp

Single-exon gene (no intron)

NCBI BLAST

NM_014380 · chrX

79 Single Exon 1 gene · 4 transcripts

FWD 5' ATGGGCCATACCCAATAGGC 3'

REV 5' ATTCCTCAACTGCAGCTCCC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 121 bp

Single-exon gene (no intron)

NCBI BLAST

NM_014380 · chrX

79 Single Exon Specific

FWD 5' CTGGTCAGAGCACCAAGCAT 3'

REV 5' GGCTGCTCCATCTCTTCGTT 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 130 bp

Single-exon gene (no intron)

NCBI BLAST

NM_014380 · chrX

78 Single Exon Specific

FWD 5' GCTCTGAATCCTGCTGGTCA 3'

REV 5' GGCTGCTCCATCTCTTCGTT 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 143 bp

Single-exon gene (no intron)

NCBI BLAST

NM_014380 · chrX

78 Single Exon Specific

FWD 5' TGAATCCTGCTGGTCAGAGC 3'

REV 5' GGCTGCTCCATCTCTTCGTT 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 139 bp

Single-exon gene (no intron)

NCBI BLAST

NM_014380 · chrX

78 Single Exon Specific

FWD 5' GAATCCTGCTGGTCAGAGCA 3'

REV 5' GGCTGCTCCATCTCTTCGTT 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 138 bp

Single-exon gene (no intron)

NCBI BLAST

NM_014380 · chrX

78 Single Exon 1 gene · 4 transcripts

FWD 5' ATGGGATGGGTGGAGATGGA 3'

REV 5' TCCTCAACTGCAGCTCCCTA 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 90 bp

Single-exon gene (no intron)

NCBI BLAST

NM_014380 · chrX

78 Single Exon Specific

FWD 5' GCTCTGAATCCTGCTGGTCA 3'

REV 5' CTGCATAGGCTGCTCCATCT 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 150 bp

Single-exon gene (no intron)

NCBI BLAST

NM_014380 · chrX

78 Single Exon Specific

FWD 5' TGAATCCTGCTGGTCAGAGC 3'

REV 5' CTGCATAGGCTGCTCCATCT 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 146 bp

Single-exon gene (no intron)

NCBI BLAST

Bex3 40 primer pairs found