Dhfr 30 primer pairs found

Human

Show all (including non-specific)

NM_001290357 · chr5

107 1 gene · 3 transcripts

FWD 5' AAGGAACCTCCACAAGGAGC 3'

REV 5' GCCACCAACTATCCAGACCA 3'

Fwd Tm 61.6°C

Rev Tm 61.4°C

GC 55%

Product 114 bp

Spans 1 intron

NM_001290357 · chr5

107 1 gene · 3 transcripts

FWD 5' AAGGAACCTCCACAAGGAGC 3'

REV 5' TGCCACCAACTATCCAGACC 3'

Fwd Tm 61.6°C

Rev Tm 61.4°C

GC 55%

Product 115 bp

Spans 1 intron

NM_001290357 · chr5

107 1 gene · 2 transcripts

FWD 5' CCACCGCTCAGGAATGAATT 3'

REV 5' CAGGAATGGAGAACCAGGTC 3'

Fwd Tm 59.9°C

Rev Tm 59.6°C

GC 50%

Product 112 bp

Spans 2 introns

NM_001290357 · chr5

107 1 gene · 3 transcripts

FWD 5' AGGAACCTCCACAAGGAGCT 3'

REV 5' TGCCTTTCTCCTCCTGGACA 3'

Fwd Tm 62.7°C

Rev Tm 62.7°C

GC 55%

Product 169 bp

Spans 2 introns

NM_001290357 · chr5

107 1 gene · 2 transcripts

FWD 5' GCCACCGCTCAGGAATGAAT 3'

REV 5' AGCTCCTTGTGGAGGTTCCT 3'

Fwd Tm 62.5°C

Rev Tm 62.7°C

GC 55%

Product 187 bp

Spans 2 introns

NM_001290357 · chr5

107 1 gene · 2 transcripts

FWD 5' CCACCGCTCAGGAATGAATT 3'

REV 5' TCAGGAATGGAGAACCAGGT 3'

Fwd Tm 59.9°C

Rev Tm 60.0°C

GC 50%

Product 113 bp

Spans 2 introns

NM_001290357 · chr5

106 1 gene · 3 transcripts

FWD 5' AAGGAACCTCCACAAGGAGC 3'

REV 5' GCCTTTCTCCTCCTGGACAT 3'

Fwd Tm 61.6°C

Rev Tm 61.2°C

GC 55%

Product 169 bp

Spans 2 introns

NM_001290357 · chr5

106 1 gene · 3 transcripts

FWD 5' AAGGAACCTCCACAAGGAGC 3'

REV 5' ATGCCTTTCTCCTCCTGGAC 3'

Fwd Tm 61.6°C

Rev Tm 61.2°C

GC 55%

Product 171 bp

Spans 2 introns

NM_001290357 · chr5

106 1 gene · 2 transcripts

FWD 5' CCACCGCTCAGGAATGAATT 3'

REV 5' CTCAGGAATGGAGAACCAGG 3'

Fwd Tm 59.9°C

Rev Tm 59.3°C

GC 50%

Product 114 bp

Spans 2 introns

NM_001290357 · chr5

106 1 gene · 2 transcripts

FWD 5' CCACCGCTCAGGAATGAATT 3'

REV 5' AGGAATGGAGAACCAGGTCT 3'

Fwd Tm 59.9°C

Rev Tm 59.8°C

GC 50%

Product 111 bp

Spans 2 introns

Previous Page 2 of 2