FER 40 primer pairs found

Human

Show all (including non-specific)

NM_001308028 · chr5

109 1 gene · 2 transcripts

FWD 5' GTTCGGGCTTGTCAGGTTCT 3'

REV 5' AGCGTGTCCATGATGAGGTG 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 129 bp

Spans 1 intron

NM_005246 · chr5

109 Specific

FWD 5' CACTGAAGAGCAGACCCGTT 3'

REV 5' ACGTGCAGTGAAGTCTAGCG 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 106 bp

Spans 1 intron

NM_001308031 · chr5

109 Specific

FWD 5' CAGCTCTGAGCCTTCCACAT 3'

REV 5' CTGAAGAGCCCAGTGCAGAT 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 131 bp

Spans 1 intron

NM_001308028 · chr5

109 1 gene · 3 transcripts

FWD 5' ATACACAGGGACCTTGCTGC 3'

REV 5' CCAAAGCTCCACACGTCACT 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 200 bp

Spans 2 introns

NM_001308031 · chr5

109 1 gene · 3 transcripts

FWD 5' ATACACAGGGACCTTGCTGC 3'

REV 5' CCAAAGCTCCACACGTCACT 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 200 bp

Spans 2 introns

NM_005246 · chr5

109 1 gene · 3 transcripts

FWD 5' ATACACAGGGACCTTGCTGC 3'

REV 5' CCAAAGCTCCACACGTCACT 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 200 bp

Spans 2 introns

NM_001308031 · chr5

109 Specific

FWD 5' GCTCTGAGCCTTCCACATCA 3'

REV 5' CTGAAGAGCCCAGTGCAGAT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 129 bp

Spans 1 intron

NM_005246 · chr5

109 Specific

FWD 5' GGCCATCACTGAAGAGCAGA 3'

REV 5' CGTGCAGTGAAGTCTAGCGA 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 111 bp

Spans 1 intron

NM_001308031 · chr5

108 Specific

FWD 5' CAGCTCTGAGCCTTCCACAT 3'

REV 5' TTGCACCATGGTACCAGTCC 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 197 bp

Spans 2 introns

NM_005246 · chr5

108 Specific

FWD 5' CACTGAAGAGCAGACCCGTT 3'

REV 5' CGTGCAGTGAAGTCTAGCGA 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 105 bp

Spans 1 intron

NM_005246 · chr5

108 Specific

FWD 5' GGCCATCACTGAAGAGCAGA 3'

REV 5' ACGTGCAGTGAAGTCTAGCG 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 112 bp

Spans 1 intron

NM_001308031 · chr5

108 Specific

FWD 5' GCTCTGAGCCTTCCACATCA 3'

REV 5' TTGCACCATGGTACCAGTCC 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 195 bp

Spans 2 introns

NM_001308028 · chr5

108 1 gene · 3 transcripts

FWD 5' CAGGGACCTTGCTGCAAGAA 3'

REV 5' AGCTCCACACGTCACTCTCT 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 191 bp

Spans 2 introns

NM_001308031 · chr5

108 1 gene · 3 transcripts

FWD 5' CAGGGACCTTGCTGCAAGAA 3'

REV 5' AGCTCCACACGTCACTCTCT 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 191 bp

Spans 2 introns

NM_001308031 · chr5

108 Specific

FWD 5' TGTTCAGCTCTGAGCCTTCC 3'

REV 5' CTGAAGAGCCCAGTGCAGAT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001308031 · chr5

108 Specific

FWD 5' GTTCAGCTCTGAGCCTTCCA 3'

REV 5' CTGAAGAGCCCAGTGCAGAT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 134 bp

Spans 1 intron

NM_001308031 · chr5

108 Specific

FWD 5' TTCAGCTCTGAGCCTTCCAC 3'

REV 5' CTGAAGAGCCCAGTGCAGAT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 133 bp

Spans 1 intron

NM_001308028 · chr5

108 1 gene · 3 transcripts

FWD 5' CACCTCATCATGGACACGCT 3'

REV 5' TTCAGGTCACTCCCAAACCC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001308028 · chr5

108 1 gene · 3 transcripts

FWD 5' CAGGGACCTTGCTGCAAGAA 3'

REV 5' CCAAAGCTCCACACGTCACT 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 195 bp

Spans 2 introns

NM_001308031 · chr5

108 1 gene · 3 transcripts

FWD 5' CAGGGACCTTGCTGCAAGAA 3'

REV 5' CCAAAGCTCCACACGTCACT 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 195 bp

Spans 2 introns

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