RSPO1 40 primer pairs found

Human

Show all (including non-specific)

NM_001242910 · chr1

115 1 gene · 4 transcripts

FWD 5' GCACGAAGCTGAGATTGTGG 3'

REV 5' GTCAGCAGGGACTACTCCAA 3'

Fwd Tm 61.1°C

Rev Tm 60.7°C

GC 55%

Product 103 bp

Spans 2 introns

NM_001242908 · chr1

115 1 gene · 4 transcripts

FWD 5' GCACGAAGCTGAGATTGTGG 3'

REV 5' GTCAGCAGGGACTACTCCAA 3'

Fwd Tm 61.1°C

Rev Tm 60.7°C

GC 55%

Product 103 bp

Spans 2 introns

NM_001242910 · chr1

115 1 gene · 4 transcripts

FWD 5' GCAGCACGAAGCTGAGATTG 3'

REV 5' CACGTCAGCAGGGACTACTC 3'

Fwd Tm 61.4°C

Rev Tm 61.7°C

GC 55%

Product 109 bp

Spans 2 introns

NM_001242908 · chr1

115 1 gene · 4 transcripts

FWD 5' GCAGCACGAAGCTGAGATTG 3'

REV 5' CACGTCAGCAGGGACTACTC 3'

Fwd Tm 61.4°C

Rev Tm 61.7°C

GC 55%

Product 109 bp

Spans 2 introns

NM_001242910 · chr1

115 1 gene · 4 transcripts

FWD 5' GCAGCACGAAGCTGAGATTG 3'

REV 5' TGTCACGTCAGCAGGGACTA 3'

Fwd Tm 61.4°C

Rev Tm 62.5°C

GC 55%

Product 112 bp

Spans 2 introns

NM_001242908 · chr1

115 1 gene · 4 transcripts

FWD 5' GCAGCACGAAGCTGAGATTG 3'

REV 5' TGTCACGTCAGCAGGGACTA 3'

Fwd Tm 61.4°C

Rev Tm 62.5°C

GC 55%

Product 112 bp

Spans 2 introns

NM_001242910 · chr1

114 1 gene · 4 transcripts

FWD 5' GCAGCACGAAGCTGAGATTG 3'

REV 5' GTCAGCAGGGACTACTCCAA 3'

Fwd Tm 61.4°C

Rev Tm 60.7°C

GC 55%

Product 106 bp

Spans 2 introns

NM_001242910 · chr1

114 1 gene · 4 transcripts

FWD 5' GCACGAAGCTGAGATTGTGG 3'

REV 5' TCACGTCAGCAGGGACTACT 3'

Fwd Tm 61.1°C

Rev Tm 62.2°C

GC 55%

Product 107 bp

Spans 2 introns

NM_001242908 · chr1

114 1 gene · 4 transcripts

FWD 5' GCAGCACGAAGCTGAGATTG 3'

REV 5' GTCAGCAGGGACTACTCCAA 3'

Fwd Tm 61.4°C

Rev Tm 60.7°C

GC 55%

Product 106 bp

Spans 2 introns

NM_001242908 · chr1

114 1 gene · 4 transcripts

FWD 5' GCACGAAGCTGAGATTGTGG 3'

REV 5' TCACGTCAGCAGGGACTACT 3'

Fwd Tm 61.1°C

Rev Tm 62.2°C

GC 55%

Product 107 bp

Spans 2 introns

NM_001038633 · chr1

109 Specific

FWD 5' AGAAGATCACCGCTGGCAAG 3'

REV 5' CCTCGACACCAAAGCGATCA 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001038633 · chr1

109 Specific

FWD 5' TGATCGCTTTGGTGTCGAGG 3'

REV 5' TCACGTCAGCAGGGACTACT 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 111 bp

Spans 1 intron

NM_001038633 · chr1

109 Specific

FWD 5' TGATCGCTTTGGTGTCGAGG 3'

REV 5' TTTGTCACGTCAGCAGGGAC 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 115 bp

Spans 1 intron

NM_001038633 · chr1

109 1 gene · 4 transcripts

FWD 5' AGCTGTTCATCCTGCTGGAG 3'

REV 5' GCCTCACAGTGCTCGATCTT 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001038633 · chr1

108 1 gene · 4 transcripts

FWD 5' GCTGTTCATCCTGCTGGAGA 3'

REV 5' GCCTCACAGTGCTCGATCTT 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001038633 · chr1

108 Specific

FWD 5' TGGTGTCGAGGCAATCAACC 3'

REV 5' TTTGTCACGTCAGCAGGGAC 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 106 bp

Spans 1 intron

NM_001038633 · chr1

108 Specific

FWD 5' GGTGTCGAGGCAATCAACCA 3'

REV 5' TTTGTCACGTCAGCAGGGAC 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 105 bp

Spans 1 intron

NM_001038633 · chr1

108 Specific

FWD 5' TGGTGTCGAGGCAATCAACC 3'

REV 5' TCACGTCAGCAGGGACTACT 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 102 bp

Spans 1 intron

NM_001038633 · chr1

108 Specific

FWD 5' GGTGTCGAGGCAATCAACCA 3'

REV 5' TCACGTCAGCAGGGACTACT 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 101 bp

Spans 1 intron

NM_001038633 · chr1

108 1 gene · 4 transcripts

FWD 5' ATCCTGCTGGAGAGGAACGA 3'

REV 5' GTTATGGCTGAAGCAGGCCT 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 144 bp

Spans 1 intron

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