Sema4f 30 primer pairs found

Human

Show all (including non-specific)

NM_004263 · chr2

118 1 gene · 3 transcripts

FWD 5' CGAGGGTTGGTCCAAGACAT 3'

REV 5' GCCACGGGAACTTCAAACAC 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 95 bp

Spans 2 introns

NM_001271661 · chr2

118 1 gene · 3 transcripts

FWD 5' CGAGGGTTGGTCCAAGACAT 3'

REV 5' GCCACGGGAACTTCAAACAC 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 95 bp

Spans 2 introns

NM_004263 · chr2

118 Specific

FWD 5' CGCAGGATTGACTGGATGGT 3'

REV 5' AAGTGAGGAGGTGAGAGGCA 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 127 bp

Spans 2 introns

NM_004263 · chr2

118 1 gene · 3 transcripts

FWD 5' CGAGGGTTGGTCCAAGACAT 3'

REV 5' CCCATGCTGAGCTTGGAGAA 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001271661 · chr2

118 1 gene · 3 transcripts

FWD 5' CGAGGGTTGGTCCAAGACAT 3'

REV 5' CCCATGCTGAGCTTGGAGAA 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 142 bp

Spans 2 introns

NM_004263 · chr2

118 1 gene · 3 transcripts

FWD 5' CGAGGGTTGGTCCAAGACAT 3'

REV 5' CTGTAGCCACGGGAACTTCA 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 100 bp

Spans 2 introns

NM_001271661 · chr2

118 1 gene · 3 transcripts

FWD 5' CGAGGGTTGGTCCAAGACAT 3'

REV 5' CTGTAGCCACGGGAACTTCA 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 100 bp

Spans 2 introns

NM_004263 · chr2

117 Specific

FWD 5' CTTCGCTTTATCCCTGCCCT 3'

REV 5' GGCAATGGCGAGAATCTGGA 3'

Fwd Tm 61.8°C

Rev Tm 62.3°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001271661 · chr2

117 Specific

FWD 5' CTTCGCTTTATCCCTGCCCT 3'

REV 5' GTCGTCCATCTCTGCTGGAG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001271661 · chr2

117 Specific

FWD 5' CGCTTTATCCCTGCCCTTCT 3'

REV 5' GTCGTCCATCTCTGCTGGAG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 144 bp

Spans 2 introns

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