ak8 30 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001020644 · chr5_KZ114864v1_alt

118 Specific

FWD 5' GTGATGCTGGAAGCTCCTGA 3'

REV 5' CCGTGCAACCTCTTCACTCT 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 123 bp

Spans 2 introns

NM_001020644 · chr5

118 Specific

FWD 5' GTGATGCTGGAAGCTCCTGA 3'

REV 5' GGTGTTTCTAAACGCCGTGC 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001020644 · chr5_KZ114864v1_alt

118 Specific

FWD 5' GTGATGCTGGAAGCTCCTGA 3'

REV 5' GGTGTTTCTAAACGCCGTGC 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001020644 · chr5

118 Specific

FWD 5' GACAAGGGCTGCACAGAGTT 3'

REV 5' CGTCTTGGGAATGGCTTCCA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001020644 · chr5_KZ115118v1_alt

118 Specific

FWD 5' GACAAGGGCTGCACAGAGTT 3'

REV 5' CGTCTTGGGAATGGCTTCCA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001020644 · chr5_KZ114864v1_alt

118 Specific

FWD 5' GACAAGGGCTGCACAGAGTT 3'

REV 5' CGTCTTGGGAATGGCTTCCA 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001020644 · chr5_KZ115118v1_alt

118 Specific

FWD 5' GTGATGCTGGAAGCTCCTGA 3'

REV 5' TCAGGCCACATGAAGGTGAC 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 104 bp

Spans 2 introns

NM_001020644 · chr5_KZ115118v1_alt

118 Specific

FWD 5' CACGTTGTGATGCTGGAAGC 3'

REV 5' CTCAGGCCACATGAAGGTGA 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 111 bp

Spans 2 introns

NM_001020644 · chr5_KZ115118v1_alt

118 Specific

FWD 5' CACGTTGTGATGCTGGAAGC 3'

REV 5' TCTCAGGCCACATGAAGGTG 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 112 bp

Spans 2 introns

NM_001020644 · chr5_KZ115118v1_alt

118 Specific

FWD 5' TGTGATGCTGGAAGCTCCTG 3'

REV 5' CTCAGGCCACATGAAGGTGA 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 106 bp

Spans 2 introns

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