Apaf1 50 primer pairs found

Human

Show all (including non-specific)

NM_181869 · chr12

109 1 gene · 5 transcripts

FWD 5' ACCGTCTCCGCATTCTGATG 3'

REV 5' AGGAACTCTCCACAGGGACT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 183 bp

Spans 2 introns

NM_181868 · chr12

109 1 gene · 5 transcripts

FWD 5' ACCGTCTCCGCATTCTGATG 3'

REV 5' AGGAACTCTCCACAGGGACT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 183 bp

Spans 2 introns

NM_181869 · chr12

109 1 gene · 5 transcripts

FWD 5' AAGACCGTCTCCGCATTCTG 3'

REV 5' AGGAACTCTCCACAGGGACT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 186 bp

Spans 2 introns

NM_181869 · chr12

108 1 gene · 5 transcripts

FWD 5' CTGATGCTTCGCAAACACCC 3'

REV 5' AGGAACTCTCCACAGGGACT 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 169 bp

Spans 2 introns

NM_181869 · chr12

108 1 gene · 5 transcripts

FWD 5' CGTCTCCGCATTCTGATGCT 3'

REV 5' AGGAACTCTCCACAGGGACT 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 181 bp

Spans 2 introns

NM_181869 · chr12

108 1 gene · 5 transcripts

FWD 5' GACCGTCTCCGCATTCTGAT 3'

REV 5' AGGAACTCTCCACAGGGACT 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 184 bp

Spans 2 introns

NM_181869 · chr12

108 Specific

FWD 5' AGTCCCTGTGGAGAGTTCCT 3'

REV 5' CCAGTGACAACGTTCCACCA 3'

Fwd Tm 61.8°C

Rev Tm 62.2°C

GC 55%

Product 139 bp

Spans 1 intron

NM_181869 · chr12

108 1 gene · 5 transcripts

FWD 5' GATCTTGCTGCCCTTCTCCA 3'

REV 5' ACAACTGGCCTCTGTGGTAC 3'

Fwd Tm 61.7°C

Rev Tm 61.5°C

GC 55%

Product 131 bp

Spans 1 intron

NM_181869 · chr12

108 1 gene · 5 transcripts

FWD 5' AGATCTTGCTGCCCTTCTCC 3'

REV 5' CCACCTTCACACAGGACTGT 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 111 bp

Spans 1 intron

NM_181869 · chr12

108 1 gene · 5 transcripts

FWD 5' AGATCTTGCTGCCCTTCTCC 3'

REV 5' ACAACTGGCCTCTGTGGTAC 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 132 bp

Spans 1 intron

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