NELFE 60 primer pairs found

Human

Show all (including non-specific)

NM_002904 · chr6_GL000255v2_alt

118 Specific

FWD 5' AGAGGAGGCTCTGCAGAAGA 3'

REV 5' CCATGACAGGCTGCTCTGAT 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 137 bp

Spans 2 introns

NM_002904 · chr6_GL000256v2_alt

118 Specific

FWD 5' AGAGGAGGCTCTGCAGAAGA 3'

REV 5' CCATGACAGGCTGCTCTGAT 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 137 bp

Spans 2 introns

NM_002904 · chr6

118 Specific

FWD 5' CCACCCAGAAACTGTGCCTT 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 2 introns

NM_002904 · chr6_GL000251v2_alt

118 Specific

FWD 5' CCACCCAGAAACTGTGCCTT 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 2 introns

NM_002904 · chr6_GL000252v2_alt

118 Specific

FWD 5' CCACCCAGAAACTGTGCCTT 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 2 introns

NM_002904 · chr6_GL000254v2_alt

118 Specific

FWD 5' CCACCCAGAAACTGTGCCTT 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 2 introns

NM_002904 · chr6_GL000255v2_alt

118 Specific

FWD 5' CCACCCAGAAACTGTGCCTT 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 2 introns

NM_002904 · chr6_GL000256v2_alt

118 Specific

FWD 5' CCACCCAGAAACTGTGCCTT 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.4°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 2 introns

NM_002904 · chr6

118 Specific

FWD 5' TTGACCTCTCCATGGACCCA 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.5°C

Rev Tm 62.0°C

GC 55%

Product 112 bp

Spans 2 introns

NM_002904 · chr6_GL000251v2_alt

118 Specific

FWD 5' TTGACCTCTCCATGGACCCA 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.5°C

Rev Tm 62.0°C

GC 55%

Product 112 bp

Spans 2 introns

NM_002904 · chr6_GL000252v2_alt

118 Specific

FWD 5' TTGACCTCTCCATGGACCCA 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.5°C

Rev Tm 62.0°C

GC 55%

Product 112 bp

Spans 2 introns

NM_002904 · chr6_GL000254v2_alt

118 Specific

FWD 5' TTGACCTCTCCATGGACCCA 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.5°C

Rev Tm 62.0°C

GC 55%

Product 112 bp

Spans 2 introns

NM_002904 · chr6_GL000255v2_alt

118 Specific

FWD 5' TTGACCTCTCCATGGACCCA 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.5°C

Rev Tm 62.0°C

GC 55%

Product 112 bp

Spans 2 introns

NM_002904 · chr6_GL000256v2_alt

118 Specific

FWD 5' TTGACCTCTCCATGGACCCA 3'

REV 5' TGTACAGACTCCACCTGGGT 3'

Fwd Tm 62.5°C

Rev Tm 62.0°C

GC 55%

Product 112 bp

Spans 2 introns

NM_002904 · chr6

116 Specific

FWD 5' CATGGACCCACCCAGAAACT 3'

REV 5' GCTGTACAGACTCCACCTGG 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 2 introns

NM_002904 · chr6_GL000251v2_alt

116 Specific

FWD 5' CATGGACCCACCCAGAAACT 3'

REV 5' GCTGTACAGACTCCACCTGG 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 2 introns

NM_002904 · chr6_GL000252v2_alt

116 Specific

FWD 5' CATGGACCCACCCAGAAACT 3'

REV 5' GCTGTACAGACTCCACCTGG 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 2 introns

NM_002904 · chr6_GL000254v2_alt

116 Specific

FWD 5' CATGGACCCACCCAGAAACT 3'

REV 5' GCTGTACAGACTCCACCTGG 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 2 introns

NM_002904 · chr6_GL000255v2_alt

116 Specific

FWD 5' CATGGACCCACCCAGAAACT 3'

REV 5' GCTGTACAGACTCCACCTGG 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 2 introns

NM_002904 · chr6_GL000256v2_alt

116 Specific

FWD 5' CATGGACCCACCCAGAAACT 3'

REV 5' GCTGTACAGACTCCACCTGG 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 2 introns

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