B52 100 primer pairs found

Drosophila

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NM_176488 · chr3R

117 1 gene · 6 transcripts

FWD 5' AGCTGGCAGGATCTCAAGGA 3'

REV 5' CAACGAGGCGAACTCAACCA 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 55%

Product 105 bp

Spans 2 introns

NM_001014619 · chr3R

117 1 gene · 6 transcripts

FWD 5' AGCTGGCAGGATCTCAAGGA 3'

REV 5' CAACGAGGCGAACTCAACCA 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 55%

Product 105 bp

Spans 2 introns

NM_176492 · chr3R

116 Specific

FWD 5' CCTCCTCTCTCTGAAAGCGC 3'

REV 5' TTCATGTCCGACAACGAGGC 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 60%

Product 132 bp

Spans 2 introns

NM_176492 · chr3R

116 Specific

FWD 5' CCTCCTCTCTCTGAAAGCGC 3'

REV 5' GCCGTCTTCATGTCCGACAA 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 60%

Product 138 bp

Spans 2 introns

NM_176492 · chr3R

116 Specific

FWD 5' CCTCCTCTCTCTGAAAGCGC 3'

REV 5' CGACAACGAGGCGAACTCAA 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 60%

Product 124 bp

Spans 2 introns

NM_176492 · chr3R

116 Specific

FWD 5' CGATGTGTGTGTCCTCCTCT 3'

REV 5' GACAACGAGGCGAACTCAAC 3'

Fwd Tm 61.0°C

Rev Tm 60.9°C

GC 55%

Product 135 bp

Spans 2 introns

NM_176492 · chr3R

115 Specific

FWD 5' CTCCTCTCTCTGAAAGCGCA 3'

REV 5' CAATGGCCGTCTTCATGTCC 3'

Fwd Tm 61.2°C

Rev Tm 60.9°C

GC 55%

Product 142 bp

Spans 2 introns

NM_176492 · chr3R

115 Specific

FWD 5' CCTCCTCTCTCTGAAAGCGC 3'

REV 5' AATGGCCGTCTTCATGTCCG 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 60%

Product 142 bp

Spans 2 introns

NM_176492 · chr3R

115 Specific

FWD 5' ACGATGTGTGTGTCCTCCTC 3'

REV 5' GACAACGAGGCGAACTCAAC 3'

Fwd Tm 61.2°C

Rev Tm 60.9°C

GC 55%

Product 136 bp

Spans 2 introns

NM_176492 · chr3R

115 Specific

FWD 5' CCTCCTCTCTCTGAAAGCGC 3'

REV 5' CAACGAGGCGAACTCAACCA 3'

Fwd Tm 62.1°C

Rev Tm 62.5°C

GC 60%

Product 121 bp

Spans 2 introns

NM_176492 · chr3R

115 Specific

FWD 5' CTCCTCTCTCTGAAAGCGCA 3'

REV 5' GACAACGAGGCGAACTCAAC 3'

Fwd Tm 61.2°C

Rev Tm 60.9°C

GC 55%

Product 122 bp

Spans 2 introns

NM_176493 · chr3R

115 1 gene · 10 transcripts

FWD 5' CAAAGAGCTGCTTGGCGAAC 3'

REV 5' GGGCCATAACGAGAGGATGA 3'

Fwd Tm 61.8°C

Rev Tm 60.9°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001170133 · chr3R

115 1 gene · 10 transcripts

FWD 5' CAAAGAGCTGCTTGGCGAAC 3'

REV 5' GGGCCATAACGAGAGGATGA 3'

Fwd Tm 61.8°C

Rev Tm 60.9°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001170132 · chr3R

115 1 gene · 10 transcripts

FWD 5' CAAAGAGCTGCTTGGCGAAC 3'

REV 5' GGGCCATAACGAGAGGATGA 3'

Fwd Tm 61.8°C

Rev Tm 60.9°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001170132 · chr3R

115 1 gene · 2 transcripts

FWD 5' GGGCGGTCGTTACAACGAAA 3'

REV 5' AGCCGAGGAGGGAATCTCTC 3'

Fwd Tm 62.7°C

Rev Tm 62.7°C

GC 55%

Product 142 bp

Spans 2 introns

NM_176493 · chr3R

114 1 gene · 10 transcripts

FWD 5' TGGCGAACGTGTGGTTGTTG 3'

REV 5' AACGGTGGGCCATAACGAGA 3'

Fwd Tm 63.5°C

Rev Tm 63.4°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001170133 · chr3R

114 1 gene · 10 transcripts

FWD 5' TGGCGAACGTGTGGTTGTTG 3'

REV 5' AACGGTGGGCCATAACGAGA 3'

Fwd Tm 63.5°C

Rev Tm 63.4°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001170132 · chr3R

114 1 gene · 10 transcripts

FWD 5' TGGCGAACGTGTGGTTGTTG 3'

REV 5' AACGGTGGGCCATAACGAGA 3'

Fwd Tm 63.5°C

Rev Tm 63.4°C

GC 55%

Product 144 bp

Spans 2 introns

NM_176493 · chr3R

114 1 gene · 10 transcripts

FWD 5' GCGAACGTGTGGTTGTTGAA 3'

REV 5' TGGGCCATAACGAGAGGATG 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 50%

Product 137 bp

Spans 2 introns

NM_001170133 · chr3R

114 1 gene · 10 transcripts

FWD 5' GCGAACGTGTGGTTGTTGAA 3'

REV 5' TGGGCCATAACGAGAGGATG 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 50%

Product 137 bp

Spans 2 introns

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