ACXE 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_144344 · chr2L

111 Specific

FWD 5' GGGACATTCACGGCTTGGAC 3'

REV 5' CCGGCACTCCAGTTGATTCT 3'

Fwd Tm 63.2°C

Rev Tm 61.9°C

GC 60%

Product 150 bp

Spans 2 introns

NM_144344 · chr2L

109 Specific

FWD 5' AACTTTGCCGGCTCCACTAG 3'

REV 5' GCGCATACGAGGTCATCACA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 142 bp

Spans 1 intron

NM_144344 · chr2L

109 Specific

FWD 5' ACCACATTATGGAGGTGCGG 3'

REV 5' CCGGCACTCCAGTTGATTCT 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 168 bp

Spans 2 introns

NM_144344 · chr2L

109 Specific

FWD 5' TTCGTTACCTCCGTGCACTG 3'

REV 5' CGCATCCTATGGAAAGGGCT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 91 bp

Spans 1 intron

NM_144344 · chr2L

109 Specific

FWD 5' TTCGTTACCTCCGTGCACTG 3'

REV 5' TGCGCATCCTATGGAAAGGG 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 93 bp

Spans 1 intron

NM_144344 · chr2L

109 Specific

FWD 5' ACATTATGGAGGTGCGGGAC 3'

REV 5' CCGGCACTCCAGTTGATTCT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 165 bp

Spans 2 introns

NM_144344 · chr2L

109 Specific

FWD 5' CACATTATGGAGGTGCGGGA 3'

REV 5' CCGGCACTCCAGTTGATTCT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 166 bp

Spans 2 introns

NM_144344 · chr2L

109 Specific

FWD 5' CATTATGGAGGTGCGGGACA 3'

REV 5' CCGGCACTCCAGTTGATTCT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 164 bp

Spans 2 introns

NM_144344 · chr2L

109 Specific

FWD 5' CGATCCCGATCACGCCAATA 3'

REV 5' CCGAATGAACTCCAATGCGC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 116 bp

Spans 1 intron

NM_144344 · chr2L

109 Specific

FWD 5' ACCTGGCTACCTCAAGGCTA 3'

REV 5' CAGTGCACGGAGGTAACGAA 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 132 bp

Spans 1 intron