ADGRF1 20 primer pairs found

Human

Show all (including non-specific)

NM_025048 · chr6

117 1 gene · 2 transcripts

FWD 5' TCTTCACCTTCACTGACGGC 3'

REV 5' TAGGTCACCTGAAGCAGCAG 3'

Fwd Tm 61.7°C

Rev Tm 61.3°C

GC 55%

Product 136 bp

Spans 2 introns

NM_153840 · chr6

117 1 gene · 2 transcripts

FWD 5' TCTTCACCTTCACTGACGGC 3'

REV 5' TAGGTCACCTGAAGCAGCAG 3'

Fwd Tm 61.7°C

Rev Tm 61.3°C

GC 55%

Product 136 bp

Spans 2 introns

NM_025048 · chr6

116 1 gene · 2 transcripts

FWD 5' TCTTCACCTTCACTGACGGC 3'

REV 5' CACCTGAAGCAGCAGCTGAT 3'

Fwd Tm 61.7°C

Rev Tm 62.5°C

GC 55%

Product 131 bp

Spans 2 introns

NM_153840 · chr6

116 1 gene · 2 transcripts

FWD 5' TCTTCACCTTCACTGACGGC 3'

REV 5' CACCTGAAGCAGCAGCTGAT 3'

Fwd Tm 61.7°C

Rev Tm 62.5°C

GC 55%

Product 131 bp

Spans 2 introns

NM_025048 · chr6

114 1 gene · 2 transcripts

FWD 5' CTTCACCTTCACTGACGGCC 3'

REV 5' CACCTGAAGCAGCAGCTGAT 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 60%

Product 130 bp

Spans 2 introns

NM_153840 · chr6

114 1 gene · 2 transcripts

FWD 5' CTTCACCTTCACTGACGGCC 3'

REV 5' CACCTGAAGCAGCAGCTGAT 3'

Fwd Tm 62.8°C

Rev Tm 62.5°C

GC 60%

Product 130 bp

Spans 2 introns

NM_025048 · chr6

113 1 gene · 2 transcripts

FWD 5' TTCACCTTCACTGACGGCCA 3'

REV 5' TCACCTGAAGCAGCAGCTGA 3'

Fwd Tm 63.6°C

Rev Tm 63.6°C

GC 55%

Product 130 bp

Spans 2 introns

NM_153840 · chr6

113 1 gene · 2 transcripts

FWD 5' TTCACCTTCACTGACGGCCA 3'

REV 5' TCACCTGAAGCAGCAGCTGA 3'

Fwd Tm 63.6°C

Rev Tm 63.6°C

GC 55%

Product 130 bp

Spans 2 introns

NM_025048 · chr6

113 1 gene · 2 transcripts

FWD 5' TTCACCTTCACTGACGGCCA 3'

REV 5' CACCTGAAGCAGCAGCTGAT 3'

Fwd Tm 63.6°C

Rev Tm 62.5°C

GC 55%

Product 129 bp

Spans 2 introns

NM_153840 · chr6

113 1 gene · 2 transcripts

FWD 5' TTCACCTTCACTGACGGCCA 3'

REV 5' CACCTGAAGCAGCAGCTGAT 3'

Fwd Tm 63.6°C

Rev Tm 62.5°C

GC 55%

Product 129 bp

Spans 2 introns

NM_025048 · chr6

112 1 gene · 2 transcripts

FWD 5' TCTTCACCTTCACTGACGGC 3'

REV 5' ACCTGAAGCAGCAGCTGATA 3'

Fwd Tm 61.7°C

Rev Tm 60.9°C

GC 55%

Product 130 bp

Spans 2 introns

NM_153840 · chr6

112 1 gene · 2 transcripts

FWD 5' TCTTCACCTTCACTGACGGC 3'

REV 5' ACCTGAAGCAGCAGCTGATA 3'

Fwd Tm 61.7°C

Rev Tm 60.9°C

GC 55%

Product 130 bp

Spans 2 introns

NM_153840 · chr6

112 Specific

FWD 5' GGACAGTAAGCTGCGACAAC 3'

REV 5' GCAGTGGGTTGAAAGGCTTT 3'

Fwd Tm 60.7°C

Rev Tm 60.9°C

GC 55%

Product 128 bp

Spans 2 introns

NM_153840 · chr6

112 Specific

FWD 5' GGACAGTAAGCTGCGACAAC 3'

REV 5' TTTGCAGTGGGTTGAAAGGC 3'

Fwd Tm 60.7°C

Rev Tm 61.2°C

GC 55%

Product 131 bp

Spans 2 introns

NM_153840 · chr6

112 Specific

FWD 5' TTGGACAGTAAGCTGCGACA 3'

REV 5' TTTGCAGTGGGTTGAAAGGC 3'

Fwd Tm 61.2°C

Rev Tm 61.2°C

GC 50%

Product 133 bp

Spans 2 introns

NM_153840 · chr6

112 Specific

FWD 5' TGGACAGTAAGCTGCGACAA 3'

REV 5' TTTGCAGTGGGTTGAAAGGC 3'

Fwd Tm 61.2°C

Rev Tm 61.2°C

GC 50%

Product 132 bp

Spans 2 introns

NM_025048 · chr6

111 1 gene · 2 transcripts

FWD 5' TTCACCTTCACTGACGGCCA 3'

REV 5' GTCACCTGAAGCAGCAGCTG 3'

Fwd Tm 63.6°C

Rev Tm 63.4°C

GC 55%

Product 131 bp

Spans 2 introns

NM_025048 · chr6

111 1 gene · 2 transcripts

FWD 5' TCTTCACCTTCACTGACGGC 3'

REV 5' ATAGGTCACCTGAAGCAGCA 3'

Fwd Tm 61.7°C

Rev Tm 60.6°C

GC 55%

Product 137 bp

Spans 2 introns

NM_025048 · chr6

110 1 gene · 2 transcripts

FWD 5' TCTTCACCTTCACTGACGGC 3'

REV 5' TCACCTGAAGCAGCAGCTGA 3'

Fwd Tm 61.7°C

Rev Tm 63.6°C

GC 55%

Product 132 bp

Spans 2 introns

NM_025048 · chr6

110 1 gene · 2 transcripts

FWD 5' CTTCACCTTCACTGACGGCC 3'

REV 5' TCACCTGAAGCAGCAGCTGA 3'

Fwd Tm 62.8°C

Rev Tm 63.6°C

GC 60%

Product 131 bp

Spans 2 introns