ANKEF1 30 primer pairs found

Human

Show all (including non-specific)

NM_022096 · chr20

119 Specific

FWD 5' TCGCCCGGAAGACTAAGAGT 3'

REV 5' CCATGCTTGACCAGCTCCTT 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 131 bp

Spans 2 introns

NM_022096 · chr20

119 1 gene · 3 transcripts

FWD 5' TCTGATCGCCCGGAAGACTA 3'

REV 5' CCATGCTTGACCAGCTCCTT 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 136 bp

Spans 2 introns

NM_022096 · chr20

117 Specific

FWD 5' AAGAGTCCCATGAAGTGGGC 3'

REV 5' CCATGCTTGACCAGCTCCTT 3'

Fwd Tm 61.7°C

Rev Tm 62.3°C

GC 55%

Product 117 bp

Spans 2 introns

NM_022096 · chr20

116 1 gene · 3 transcripts

FWD 5' TTCTGATCGCCCGGAAGACT 3'

REV 5' AAGCCATGCTTGACCAGCTC 3'

Fwd Tm 62.9°C

Rev Tm 62.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_022096 · chr20

116 Specific

FWD 5' TCGCCCGGAAGACTAAGAGT 3'

REV 5' AAGCCATGCTTGACCAGCTC 3'

Fwd Tm 62.3°C

Rev Tm 62.8°C

GC 55%

Product 134 bp

Spans 2 introns

NM_022096 · chr20

116 Specific

FWD 5' TCGCCCGGAAGACTAAGAGT 3'

REV 5' AAAGCCATGCTTGACCAGCT 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 135 bp

Spans 2 introns

NM_022096 · chr20

116 1 gene · 3 transcripts

FWD 5' TCTGATCGCCCGGAAGACTA 3'

REV 5' AAGCCATGCTTGACCAGCTC 3'

Fwd Tm 62.1°C

Rev Tm 62.8°C

GC 55%

Product 139 bp

Spans 2 introns

NM_022096 · chr20

116 1 gene · 3 transcripts

FWD 5' CTTCTGATCGCCCGGAAGAC 3'

REV 5' CCATGCTTGACCAGCTCCTT 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 60%

Product 138 bp

Spans 2 introns

NM_022096 · chr20

116 1 gene · 3 transcripts

FWD 5' TCTGATCGCCCGGAAGACTA 3'

REV 5' AAAGCCATGCTTGACCAGCT 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 140 bp

Spans 2 introns

NM_022096 · chr20

116 1 gene · 3 transcripts

FWD 5' TTCTGATCGCCCGGAAGACT 3'

REV 5' CCATGCTTGACCAGCTCCTT 3'

Fwd Tm 62.9°C

Rev Tm 62.3°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001303472 · chr20

109 Specific

FWD 5' AGTCCTCAATGCCGTATGCC 3'

REV 5' TTCAAGGGCGATCAGAGCAC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001303472 · chr20

109 Specific

FWD 5' AGTCCTCAATGCCGTATGCC 3'

REV 5' GTTCAAGGGCGATCAGAGCA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001303472 · chr20

109 Specific

FWD 5' AGTCCTCAATGCCGTATGCC 3'

REV 5' TGTTCAAGGGCGATCAGAGC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 101 bp

Spans 1 intron

NM_001303472 · chr20

109 Specific

FWD 5' TCCTCAATGCCGTATGCCAG 3'

REV 5' TTCAAGGGCGATCAGAGCAC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 97 bp

Spans 1 intron

NM_001303472 · chr20

109 Specific

FWD 5' TCCTCAATGCCGTATGCCAG 3'

REV 5' GTTCAAGGGCGATCAGAGCA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001303472 · chr20

109 Specific

FWD 5' TCCTCAATGCCGTATGCCAG 3'

REV 5' TGTTCAAGGGCGATCAGAGC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001303472 · chr20

109 Specific

FWD 5' TCCTCAATGCCGTATGCCAG 3'

REV 5' GCGATCAGAGCACAGCGATA 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 90 bp

Spans 1 intron

NM_001303472 · chr20

109 Specific

FWD 5' AGTCCTCAATGCCGTATGCC 3'

REV 5' GCGATCAGAGCACAGCGATA 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 92 bp

Spans 1 intron

NM_001303472 · chr20

109 Specific

FWD 5' AGGCATGTGTACTCAGTCCT 3'

REV 5' CGATAATGCCGCTTAGTCGG 3'

Fwd Tm 60.0°C

Rev Tm 60.4°C

GC 50%

Product 91 bp

Spans 2 introns

NM_001303472 · chr20

109 Specific

FWD 5' GGCATGTGTACTCAGTCCTC 3'

REV 5' CGATAATGCCGCTTAGTCGG 3'

Fwd Tm 59.5°C

Rev Tm 60.4°C

GC 55%

Product 90 bp

Spans 2 introns

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