Ac3 20 primer pairs found

Drosophila

Show all (including non-specific)

NM_136272 · chr2L

119 1 gene · 2 transcripts

FWD 5' GCGTGCAAACTTGCTAGCAG 3'

REV 5' GCATCCTCCGTCCACTCAAA 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001014496 · chr2L

119 1 gene · 2 transcripts

FWD 5' GCGTGCAAACTTGCTAGCAG 3'

REV 5' GCATCCTCCGTCCACTCAAA 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 147 bp

Spans 2 introns

NM_136272 · chr2L

118 Specific

FWD 5' CCATTGCAGCTCTGCATTGC 3'

REV 5' CCAAGGTGCTTAACGCCAGA 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 150 bp

Spans 2 introns

NM_136272 · chr2L

118 Specific

FWD 5' CCATTGCAGCTCTGCATTGC 3'

REV 5' TGAAGCGGCATATCGCGTAG 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 110 bp

Spans 2 introns

NM_136272 · chr2L

118 Specific

FWD 5' CCATTGCAGCTCTGCATTGC 3'

REV 5' CTGAAGCGGCATATCGCGTA 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 111 bp

Spans 2 introns

NM_136272 · chr2L

118 Specific

FWD 5' CCATTGCAGCTCTGCATTGC 3'

REV 5' GAAGCGGCATATCGCGTAGA 3'

Fwd Tm 62.3°C

Rev Tm 61.9°C

GC 55%

Product 109 bp

Spans 2 introns

NM_136272 · chr2L

117 Specific

FWD 5' CCATTGCAGCTCTGCATTGC 3'

REV 5' AAGGTGCTTAACGCCAGAGC 3'

Fwd Tm 62.3°C

Rev Tm 62.6°C

GC 55%

Product 148 bp

Spans 2 introns

NM_136272 · chr2L

117 Specific

FWD 5' TGTCCATTGCAGCTCTGCAT 3'

REV 5' TGAAGCGGCATATCGCGTAG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 50%

Product 113 bp

Spans 2 introns

NM_136272 · chr2L

117 Specific

FWD 5' TGTCCATTGCAGCTCTGCAT 3'

REV 5' CTGAAGCGGCATATCGCGTA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 50%

Product 114 bp

Spans 2 introns

NM_136272 · chr2L

117 Specific

FWD 5' TGTCCATTGCAGCTCTGCAT 3'

REV 5' GAAGCGGCATATCGCGTAGA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 50%

Product 112 bp

Spans 2 introns

NM_136272 · chr2L

117 Specific

FWD 5' TTGTCCATTGCAGCTCTGCA 3'

REV 5' TGAAGCGGCATATCGCGTAG 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 50%

Product 114 bp

Spans 2 introns

NM_001014496 · chr2L

116 1 gene · 2 transcripts

FWD 5' GCGTGCAAACTTGCTAGCAG 3'

REV 5' TAAATGCACTGCGGAAGCGT 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 126 bp

Spans 2 introns

NM_001014496 · chr2L

116 1 gene · 2 transcripts

FWD 5' TTGCGTGCAAACTTGCTAGC 3'

REV 5' GCATCCTCCGTCCACTCAAA 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 50%

Product 149 bp

Spans 2 introns

NM_001014496 · chr2L

114 1 gene · 2 transcripts

FWD 5' GCGTGCAAACTTGCTAGCAG 3'

REV 5' TGCATCCTCCGTCCACTCAA 3'

Fwd Tm 62.1°C

Rev Tm 63.1°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001014496 · chr2L

113 1 gene · 2 transcripts

FWD 5' CGGTGTCTTGCGTGCAAACT 3'

REV 5' AAATGCACTGCGGAAGCGTC 3'

Fwd Tm 63.5°C

Rev Tm 63.6°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001014496 · chr2L

113 1 gene · 2 transcripts

FWD 5' TCGGTGTCTTGCGTGCAAAC 3'

REV 5' AAATGCACTGCGGAAGCGTC 3'

Fwd Tm 63.5°C

Rev Tm 63.6°C

GC 55%

Product 135 bp

Spans 2 introns

NM_001014496 · chr2L

113 1 gene · 2 transcripts

FWD 5' CTCGGTGTCTTGCGTGCAAA 3'

REV 5' AAATGCACTGCGGAAGCGTC 3'

Fwd Tm 63.2°C

Rev Tm 63.6°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001014496 · chr2L

113 1 gene · 2 transcripts

FWD 5' ATTGCTCGGTGTCTTGCGTG 3'

REV 5' AAATGCACTGCGGAAGCGTC 3'

Fwd Tm 63.1°C

Rev Tm 63.6°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001014496 · chr2L

113 1 gene · 2 transcripts

FWD 5' GCGTGCAAACTTGCTAGCAG 3'

REV 5' TTAAATGCACTGCGGAAGCG 3'

Fwd Tm 62.1°C

Rev Tm 61.1°C

GC 55%

Product 127 bp

Spans 2 introns

NM_001014496 · chr2L

113 1 gene · 2 transcripts

FWD 5' TGCGTGCAAACTTGCTAGCA 3'

REV 5' GCATCCTCCGTCCACTCAAA 3'

Fwd Tm 62.8°C

Rev Tm 61.9°C

GC 50%

Product 148 bp

Spans 2 introns