Acf 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_080486 · chr3R

109 Specific

FWD 5' TGTGTTCACCTCAAGCGACC 3'

REV 5' CATCGCGGTCATGATCACCA 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 115 bp

Spans 1 intron

NM_080486 · chr3R

109 Specific

FWD 5' CATCCTTAACCGCCGGTGAA 3'

REV 5' GGTCGCTTGAGGTGAACACA 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 145 bp

Spans 1 intron

NM_080486 · chr3R

109 Specific

FWD 5' GCTAAACGAGGAGCTGCTCA 3'

REV 5' AGCTGCATGTCCCTACATCG 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 138 bp

Spans 1 intron

NM_080486 · chr3R

109 Specific

FWD 5' TAAACGAGGAGCTGCTCAGC 3'

REV 5' AGCTGCATGTCCCTACATCG 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 136 bp

Spans 1 intron

NM_080486 · chr3R

109 Specific

FWD 5' CAGCTAAACGAGGAGCTGCT 3'

REV 5' AGCTGCATGTCCCTACATCG 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 140 bp

Spans 1 intron

NM_080486 · chr3R

109 Specific

FWD 5' GAGCTGCTCAGCGACATTCA 3'

REV 5' GGCCTAAACGGTAGCTGCAT 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 140 bp

Spans 1 intron

NM_080486 · chr3R

109 Specific

FWD 5' GCTAAACGAGGAGCTGCTCA 3'

REV 5' GCTGCATGTCCCTACATCGA 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 137 bp

Spans 1 intron

NM_080486 · chr3R

109 Specific

FWD 5' TAAACGAGGAGCTGCTCAGC 3'

REV 5' GCTGCATGTCCCTACATCGA 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 135 bp

Spans 1 intron

NM_080486 · chr3R

109 Specific

FWD 5' CAGCTAAACGAGGAGCTGCT 3'

REV 5' GCTGCATGTCCCTACATCGA 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 139 bp

Spans 1 intron

NM_080486 · chr3R

108 Specific

FWD 5' AGCTGCTCAGCGACATTCAG 3'

REV 5' GGCCTAAACGGTAGCTGCAT 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 139 bp

Spans 1 intron