Ack 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_139602 · chr3L

109 Specific

FWD 5' GTCCACCGGACGTTTATGCT 3'

REV 5' GAGGCGACATACTGGCCAAA 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 105 bp

Spans 1 intron

NM_139602 · chr3L

109 Specific

FWD 5' TGTCCACCGGACGTTTATGC 3'

REV 5' GAGGCGACATACTGGCCAAA 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 106 bp

Spans 1 intron

NM_139602 · chr3L

108 Specific

FWD 5' CCTGTCCACCGGACGTTTAT 3'

REV 5' TTGGACTCGTGGTGACTTCG 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 145 bp

Spans 1 intron

NM_139602 · chr3L

108 Specific

FWD 5' CCTGTCCACCGGACGTTTAT 3'

REV 5' GCATCACAGGAGGCGACATA 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 117 bp

Spans 1 intron

NM_139602 · chr3L

108 Specific

FWD 5' GTCCACCGGACGTTTATGCT 3'

REV 5' GCATCACAGGAGGCGACATA 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 114 bp

Spans 1 intron

NM_139602 · chr3L

108 Specific

FWD 5' CCTGTCCACCGGACGTTTAT 3'

REV 5' CCTTGGACTCGTGGTGACTT 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 147 bp

Spans 1 intron

NM_139602 · chr3L

108 Specific

FWD 5' GTCCACCGGACGTTTATGCT 3'

REV 5' TTGGACTCGTGGTGACTTCG 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 142 bp

Spans 1 intron

NM_139602 · chr3L

108 Specific

FWD 5' TTAGCCAAGTGCAGCGACTG 3'

REV 5' CGCCGAAGTGGAGGTCATTT 3'

Fwd Tm 62.6°C

Rev Tm 62.4°C

GC 55%

Product 109 bp

Spans 1 intron

NM_139602 · chr3L

107 Specific

FWD 5' AAATCGATCGCGAAGGCGAG 3'

REV 5' GAGGCGACATACTGGCCAAA 3'

Fwd Tm 62.6°C

Rev Tm 62.2°C

GC 55%

Product 147 bp

Spans 1 intron

NM_139602 · chr3L

107 Specific

FWD 5' GTCCACCGGACGTTTATGCT 3'

REV 5' CACAGGAGGCGACATACTGG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 110 bp

Spans 1 intron