Acp53Ea 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_057803 · chr2R

105 Specific

FWD 5' TGGCTCTCGTATTTGTGGCC 3'

REV 5' TCTCTCAGCAGACTGGCAAG 3'

Fwd Tm 62.2°C

Rev Tm 61.3°C

GC 55%

Product 106 bp

Spans 1 intron

NM_057803 · chr2R

105 Specific

FWD 5' GCTCTCGTATTTGTGGCCCA 3'

REV 5' TCTCTCAGCAGACTGGCAAG 3'

Fwd Tm 62.2°C

Rev Tm 61.3°C

GC 55%

Product 104 bp

Spans 1 intron

NM_057803 · chr2R

105 Specific

FWD 5' TGGCTCTCGTATTTGTGGCC 3'

REV 5' TGGCAAGCGCTTTAGTACCA 3'

Fwd Tm 62.2°C

Rev Tm 61.6°C

GC 55%

Product 93 bp

Spans 1 intron

NM_057803 · chr2R

105 Specific

FWD 5' GCTCTCGTATTTGTGGCCCA 3'

REV 5' TGGCAAGCGCTTTAGTACCA 3'

Fwd Tm 62.2°C

Rev Tm 61.6°C

GC 55%

Product 91 bp

Spans 1 intron

NM_057803 · chr2R

105 Specific

FWD 5' TGGCTCTCGTATTTGTGGCC 3'

REV 5' AGACTGGCAAGCGCTTTAGT 3'

Fwd Tm 62.2°C

Rev Tm 61.6°C

GC 55%

Product 97 bp

Spans 1 intron

NM_057803 · chr2R

105 Specific

FWD 5' GCTCTCGTATTTGTGGCCCA 3'

REV 5' AGACTGGCAAGCGCTTTAGT 3'

Fwd Tm 62.2°C

Rev Tm 61.6°C

GC 55%

Product 95 bp

Spans 1 intron

NM_057803 · chr2R

105 Specific

FWD 5' TGGCTCTCGTATTTGTGGCC 3'

REV 5' CAGACTGGCAAGCGCTTTAG 3'

Fwd Tm 62.2°C

Rev Tm 61.1°C

GC 55%

Product 98 bp

Spans 1 intron

NM_057803 · chr2R

105 Specific

FWD 5' GCTCTCGTATTTGTGGCCCA 3'

REV 5' CAGACTGGCAAGCGCTTTAG 3'

Fwd Tm 62.2°C

Rev Tm 61.1°C

GC 55%

Product 96 bp

Spans 1 intron

NM_057803 · chr2R

104 Specific

FWD 5' TGGCTCTCGTATTTGTGGCC 3'

REV 5' GCAGACTGGCAAGCGCTTTA 3'

Fwd Tm 62.2°C

Rev Tm 63.1°C

GC 55%

Product 99 bp

Spans 1 intron

NM_057803 · chr2R

104 Specific

FWD 5' GCTCTCGTATTTGTGGCCCA 3'

REV 5' GCAGACTGGCAAGCGCTTTA 3'

Fwd Tm 62.2°C

Rev Tm 63.1°C

GC 55%

Product 97 bp

Spans 1 intron