Act88F 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_079643 · chr3R

109 Specific

FWD 5' AGGAATCGAACGTGCGACTC 3'

REV 5' GCCCGATCCGTTGTCGATAA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 117 bp

Spans 1 intron

NM_079643 · chr3R

109 Specific

FWD 5' GGAATCGAACGTGCGACTCT 3'

REV 5' GCCCGATCCGTTGTCGATAA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 116 bp

Spans 1 intron

NM_079643 · chr3R

109 Specific

FWD 5' TCATGAAGTGCGACGTGGAC 3'

REV 5' CCTTCTGCATACGATCGGCA 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 102 bp

Spans 1 intron

NM_079643 · chr3R

109 Specific

FWD 5' TGAAGTGCGACGTGGACATC 3'

REV 5' CCTTCTGCATACGATCGGCA 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 99 bp

Spans 1 intron

NM_079643 · chr3R

109 Specific

FWD 5' GTGGACATCCGCAAGGATCT 3'

REV 5' GGCAGTGATCTCCTTCTGCA 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 99 bp

Spans 1 intron

NM_079643 · chr3R

109 Specific

FWD 5' TGGACATCCGCAAGGATCTG 3'

REV 5' GGCAGTGATCTCCTTCTGCA 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 98 bp

Spans 1 intron

NM_079643 · chr3R

109 Specific

FWD 5' GGACATCCGCAAGGATCTGT 3'

REV 5' GGCAGTGATCTCCTTCTGCA 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 97 bp

Spans 1 intron

NM_079643 · chr3R

108 Specific

FWD 5' TCCGCAAGGATCTGTATGCC 3'

REV 5' GGCAGTGATCTCCTTCTGCA 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 92 bp

Spans 1 intron

NM_079643 · chr3R

108 Specific

FWD 5' CAGGAATCGAACGTGCGACT 3'

REV 5' GCCCGATCCGTTGTCGATAA 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 118 bp

Spans 1 intron

NM_079643 · chr3R

108 Specific

FWD 5' ACAGGAATCGAACGTGCGAC 3'

REV 5' CCCGCATCATCGTCACACAT 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 91 bp

Spans 1 intron