Acta1 10 primer pairs found

Human

Show all (including non-specific)

NM_001100 · chr1

109 Specific

FWD 5' TTGAGACCTTCAACGTGCCC 3'

REV 5' CAGCGCGTAGCCCTCATAAA 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 143 bp

Spans 1 intron

NM_001100 · chr1

109 Specific

FWD 5' TTGAGACCTTCAACGTGCCC 3'

REV 5' AGCCCTCATAAATGGGCACG 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001100 · chr1

108 Specific

FWD 5' CAGAAAGAGATCACCGCGCT 3'

REV 5' TGGTGATCCACATCTGCTGG 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001100 · chr1

108 Specific

FWD 5' AGAAAGAGATCACCGCGCTG 3'

REV 5' TGGTGATCCACATCTGCTGG 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001100 · chr1

108 Specific

FWD 5' CCGCCCAGAAACTAGACACA 3'

REV 5' GTCGCCCACGTAGGAATCTT 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 194 bp

Spans 2 introns

NM_001100 · chr1

108 Specific

FWD 5' CAGAAAGAGATCACCGCGCT 3'

REV 5' ACATCTGCTGGAAGGTGGAC 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 127 bp

Spans 1 intron

NM_001100 · chr1

108 Specific

FWD 5' CAGAAAGAGATCACCGCGCT 3'

REV 5' ACTCCTGCTTGGTGATCCAC 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001100 · chr1

108 Specific

FWD 5' AGAAAGAGATCACCGCGCTG 3'

REV 5' ACATCTGCTGGAAGGTGGAC 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001100 · chr1

108 Specific

FWD 5' AGAAAGAGATCACCGCGCTG 3'

REV 5' ACTCCTGCTTGGTGATCCAC 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 144 bp

Spans 1 intron

NM_001100 · chr1

108 Specific

FWD 5' CAGAAAGAGATCACCGCGCT 3'

REV 5' CATCTGCTGGAAGGTGGACA 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 126 bp

Spans 1 intron