Actbeta 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_143685 · chr4

110 Specific

FWD 5' CACCTTGTGCCATGAAAGCG 3'

REV 5' CATCGCTGGGTGCAATGTTG 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 1 intron

NM_143685 · chr4

109 Specific

FWD 5' GTGCCATGAAAGCGGAAAGC 3'

REV 5' CATCGCTGGGTGCAATGTTG 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 143 bp

Spans 1 intron

NM_143685 · chr4

109 Specific

FWD 5' GCACCTTGTGCCATGAAAGC 3'

REV 5' CATCGCTGGGTGCAATGTTG 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 150 bp

Spans 1 intron

NM_143685 · chr4

107 Specific

FWD 5' GTGCCATGAAAGCGGAAAGC 3'

REV 5' GAACTTCATCGCTGGGTGCA 3'

Fwd Tm 62.2°C

Rev Tm 62.6°C

GC 55%

Product 149 bp

Spans 1 intron

NM_143685 · chr4

107 Specific

FWD 5' GTGCCATGAAAGCGGAAAGC 3'

REV 5' AACTTCATCGCTGGGTGCAA 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 148 bp

Spans 1 intron

NM_143685 · chr4

107 Specific

FWD 5' GTGCCATGAAAGCGGAAAGC 3'

REV 5' TCATCGCTGGGTGCAATGTT 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 144 bp

Spans 1 intron

NM_143685 · chr4

107 Specific

FWD 5' GTGCCATGAAAGCGGAAAGC 3'

REV 5' TTCATCGCTGGGTGCAATGT 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 145 bp

Spans 1 intron

NM_143685 · chr4

106 Specific

FWD 5' GTGCCATGAAAGCGGAAAGC 3'

REV 5' ACTTCATCGCTGGGTGCAAT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 147 bp

Spans 1 intron

NM_143685 · chr4

106 Specific

FWD 5' GTGCCATGAAAGCGGAAAGC 3'

REV 5' CTTCATCGCTGGGTGCAATG 3'

Fwd Tm 62.2°C

Rev Tm 61.5°C

GC 55%

Product 146 bp

Spans 1 intron

NM_143685 · chr4

106 Specific

FWD 5' CACCTTGTGCCATGAAAGCG 3'

REV 5' TCATCGCTGGGTGCAATGTT 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 150 bp

Spans 1 intron