No gene-specific primers found for Ada1-1. Showing all 20 primers including those that may amplify other genes. Consider validating with NCBI Primer-BLAST.

Ada1-1 20 primer pairs found

Drosophila

Show all (including non-specific)

NM_164984 · chr2L

109 2 genes · 3 transcripts

FWD 5' TGCGAAGAAGTCCAACCCAC 3'

REV 5' ATGCGAGCCTATCAGGTTGC 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 90 bp

Spans 1 intron

NCBI BLAST

NM_164984 · chr2L

109 2 genes · 3 transcripts

FWD 5' TGCGAAGAAGTCCAACCCAC 3'

REV 5' GCATGCGAGCCTATCAGGTT 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 92 bp

Spans 1 intron

NCBI BLAST

NM_001298929 · chr2L

109 2 genes · 3 transcripts

FWD 5' TGCGAAGAAGTCCAACCCAC 3'

REV 5' ATGCGAGCCTATCAGGTTGC 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 90 bp

Spans 1 intron

NCBI BLAST

NM_001298929 · chr2L

109 2 genes · 3 transcripts

FWD 5' TGCGAAGAAGTCCAACCCAC 3'

REV 5' GCATGCGAGCCTATCAGGTT 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 92 bp

Spans 1 intron

NCBI BLAST

NM_164984 · chr2L

109 2 genes · 3 transcripts

FWD 5' GCGACAGAACGACGATGTGA 3'

REV 5' ATGCGAGCCTATCAGGTTGC 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 124 bp

Spans 1 intron

NCBI BLAST

NM_164984 · chr2L

109 2 genes · 3 transcripts

FWD 5' GCGACAGAACGACGATGTGA 3'

REV 5' GCATGCGAGCCTATCAGGTT 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 126 bp

Spans 1 intron

NCBI BLAST

NM_001298929 · chr2L

109 2 genes · 3 transcripts

FWD 5' GCGACAGAACGACGATGTGA 3'

REV 5' ATGCGAGCCTATCAGGTTGC 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 124 bp

Spans 1 intron

NCBI BLAST

NM_001298929 · chr2L

109 2 genes · 3 transcripts

FWD 5' GCGACAGAACGACGATGTGA 3'

REV 5' GCATGCGAGCCTATCAGGTT 3'

Fwd Tm 62.1°C

Rev Tm 62.3°C

GC 55%

Product 126 bp

Spans 1 intron

NCBI BLAST

NM_164984 · chr2L

109 2 genes · 3 transcripts

FWD 5' CTGCGAAGAAGTCCAACCCA 3'

REV 5' ATGCGAGCCTATCAGGTTGC 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 91 bp

Spans 1 intron

NCBI BLAST

NM_164984 · chr2L

109 2 genes · 3 transcripts

FWD 5' CTGCGAAGAAGTCCAACCCA 3'

REV 5' GCATGCGAGCCTATCAGGTT 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 93 bp

Spans 1 intron

NCBI BLAST

NM_001298929 · chr2L

109 2 genes · 3 transcripts

FWD 5' CTGCGAAGAAGTCCAACCCA 3'

REV 5' ATGCGAGCCTATCAGGTTGC 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 91 bp

Spans 1 intron

NCBI BLAST

NM_001298929 · chr2L

109 2 genes · 3 transcripts

FWD 5' CTGCGAAGAAGTCCAACCCA 3'

REV 5' GCATGCGAGCCTATCAGGTT 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 93 bp

Spans 1 intron

NCBI BLAST

NM_164984 · chr2L

108 2 genes · 3 transcripts

FWD 5' CGCTGGAACTGGACAAGGAA 3'

REV 5' ATTACTGTGCGTTCGGTGGG 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 112 bp

Spans 1 intron

NCBI BLAST

NM_001298929 · chr2L

108 2 genes · 3 transcripts

FWD 5' CGCTGGAACTGGACAAGGAA 3'

REV 5' ATTACTGTGCGTTCGGTGGG 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 112 bp

Spans 1 intron

NCBI BLAST

NM_164984 · chr2L

108 2 genes · 3 transcripts

FWD 5' CGCTGGAACTGGACAAGGAA 3'

REV 5' GGCAGTCCTTGAGGGTGATT 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 55%

Product 129 bp

Spans 1 intron

NCBI BLAST

NM_001298929 · chr2L

108 2 genes · 3 transcripts

FWD 5' CGCTGGAACTGGACAAGGAA 3'

REV 5' GGCAGTCCTTGAGGGTGATT 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 55%

Product 129 bp

Spans 1 intron

NCBI BLAST

NM_164984 · chr2L

108 2 genes · 3 transcripts

FWD 5' TTATGCGGCGACAGAACGAC 3'

REV 5' ATGCGAGCCTATCAGGTTGC 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 131 bp

Spans 1 intron

NCBI BLAST

NM_164984 · chr2L

108 2 genes · 3 transcripts

FWD 5' TTATGCGGCGACAGAACGAC 3'

REV 5' GCATGCGAGCCTATCAGGTT 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 133 bp

Spans 1 intron

NCBI BLAST

NM_001298929 · chr2L

108 2 genes · 3 transcripts

FWD 5' TTATGCGGCGACAGAACGAC 3'

REV 5' ATGCGAGCCTATCAGGTTGC 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 131 bp

Spans 1 intron

NCBI BLAST

NM_001298929 · chr2L

108 2 genes · 3 transcripts

FWD 5' TTATGCGGCGACAGAACGAC 3'

REV 5' GCATGCGAGCCTATCAGGTT 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 133 bp

Spans 1 intron

NCBI BLAST