Adgf-A 20 primer pairs found

Drosophila

Show all (including non-specific)

NM_206397 · chr3L

110 Specific

FWD 5' TGTGTGGTCTACGCTTGTGG 3'

REV 5' TTAGGGTCCGATCGCGACTA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 104 bp

Spans 1 intron

NM_206397 · chr3L

110 Specific

FWD 5' TGTGTGGTCTACGCTTGTGG 3'

REV 5' CGGATGACTGGCGACATGAT 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 139 bp

Spans 1 intron

NM_206397 · chr3L

110 Specific

FWD 5' TGTGTGGTCTACGCTTGTGG 3'

REV 5' ATCGCGACTAGGTCCACTCT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 94 bp

Spans 1 intron

NM_079406 · chr3L

110 Specific

FWD 5' GCTTGTGATAGGGCGCTACA 3'

REV 5' CGGATGACTGGCGACATGAT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 131 bp

Spans 1 intron

NM_079406 · chr3L

110 Specific

FWD 5' GCTTGTGATAGGGCGCTACA 3'

REV 5' TTAGGGTCCGATCGCGACTA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 96 bp

Spans 1 intron

NM_206397 · chr3L

109 1 gene · 2 transcripts

FWD 5' GAATCAGTCGTTGGGAGGCA 3'

REV 5' GCGATTGAGGAGCTCCGATT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 1 intron

NM_079406 · chr3L

109 1 gene · 2 transcripts

FWD 5' GAATCAGTCGTTGGGAGGCA 3'

REV 5' GCGATTGAGGAGCTCCGATT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 140 bp

Spans 1 intron

NM_206397 · chr3L

109 1 gene · 2 transcripts

FWD 5' AATCAGTCGTTGGGAGGCAC 3'

REV 5' GCGATTGAGGAGCTCCGATT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 1 intron

NM_206397 · chr3L

109 Specific

FWD 5' TGTGTGGTCTACGCTTGTGG 3'

REV 5' TGACTGGCGACATGATGAGC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 135 bp

Spans 1 intron

NM_079406 · chr3L

109 1 gene · 2 transcripts

FWD 5' AATCAGTCGTTGGGAGGCAC 3'

REV 5' GCGATTGAGGAGCTCCGATT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 139 bp

Spans 1 intron

NM_079406 · chr3L

109 Specific

FWD 5' GCTTGTGATAGGGCGCTACA 3'

REV 5' TTTCGTTAGGGTCCGATCGC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 101 bp

Spans 1 intron

NM_206397 · chr3L

109 Specific

FWD 5' TGTGTGGTCTACGCTTGTGG 3'

REV 5' TTTCGTTAGGGTCCGATCGC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 109 bp

Spans 1 intron

NM_079406 · chr3L

109 Specific

FWD 5' GCTTGTGATAGGGCGCTACA 3'

REV 5' TGACTGGCGACATGATGAGC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 1 intron

NM_079406 · chr3L

109 Specific

FWD 5' GCTTGTGATAGGGCGCTACA 3'

REV 5' AGGCGATAAGATTACGGCGG 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 148 bp

Spans 1 intron

NM_079406 · chr3L

108 Specific

FWD 5' GCTTGTGATAGGGCGCTACA 3'

REV 5' GGCGATAAGATTACGGCGGA 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 147 bp

Spans 1 intron

NM_206397 · chr3L

108 Specific

FWD 5' CTTGTGGGCGCTACATCGAA 3'

REV 5' TGACTGGCGACATGATGAGC 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 122 bp

Spans 1 intron

NM_206397 · chr3L

108 Specific

FWD 5' CTTGTGGGCGCTACATCGAA 3'

REV 5' TTAGGGTCCGATCGCGACTA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 91 bp

Spans 1 intron

NM_079406 · chr3L

108 Specific

FWD 5' GAATCAGTCGTTGGGAGGCA 3'

REV 5' GGTTTCGATGTAGCGCCCTA 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 118 bp

Spans 1 intron

NM_206397 · chr3L

108 1 gene · 2 transcripts

FWD 5' CAGTCGTTGGGAGGCACTTT 3'

REV 5' GCGATTGAGGAGCTCCGATT 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 132 bp

Spans 1 intron

NM_079406 · chr3L

108 1 gene · 2 transcripts

FWD 5' CAGTCGTTGGGAGGCACTTT 3'

REV 5' GCGATTGAGGAGCTCCGATT 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 1 intron