Aff4 10 primer pairs found

Human

Show all (including non-specific)

NM_014423 · chr5

117 Specific

FWD 5' TACTTTGCCTGCGATGCGAG 3'

REV 5' CAGGCGATGGTGCTTGAGAA 3'

Fwd Tm 62.6°C

Rev Tm 62.4°C

GC 55%

Product 135 bp

Spans 2 introns

NM_014423 · chr5

117 Specific

FWD 5' GGCAACAGCATGACTGAGCT 3'

REV 5' GGAGGCCATGAATGCGTCAT 3'

Fwd Tm 62.7°C

Rev Tm 62.6°C

GC 55%

Product 146 bp

Spans 2 introns

NM_014423 · chr5

117 Specific

FWD 5' AGCATGACTGAGCTGAAGCC 3'

REV 5' GGAGGCCATGAATGCGTCAT 3'

Fwd Tm 62.2°C

Rev Tm 62.6°C

GC 55%

Product 140 bp

Spans 2 introns

NM_014423 · chr5

117 Specific

FWD 5' AGACACTGACAGAGCACCTG 3'

REV 5' CTGAATTGCCTGGTGACAGC 3'

Fwd Tm 61.2°C

Rev Tm 61.2°C

GC 55%

Product 123 bp

Spans 2 introns

NM_014423 · chr5

117 Specific

FWD 5' GACACTGACAGAGCACCTGA 3'

REV 5' CTGAATTGCCTGGTGACAGC 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 122 bp

Spans 2 introns

NM_014423 · chr5

116 Specific

FWD 5' CATGACTGAGCTGAAGCCCA 3'

REV 5' GGAGGCCATGAATGCGTCAT 3'

Fwd Tm 61.9°C

Rev Tm 62.6°C

GC 55%

Product 138 bp

Spans 2 introns

NM_014423 · chr5

116 Specific

FWD 5' AGACACTGACAGAGCACCTG 3'

REV 5' CCTGGTGACAGCTTTGGAGA 3'

Fwd Tm 61.2°C

Rev Tm 61.6°C

GC 55%

Product 115 bp

Spans 2 introns

NM_014423 · chr5

116 Specific

FWD 5' GTACTTTGCCTGCGATGCGA 3'

REV 5' CAGGCGATGGTGCTTGAGAA 3'

Fwd Tm 62.8°C

Rev Tm 62.4°C

GC 55%

Product 136 bp

Spans 2 introns

NM_014423 · chr5

116 Specific

FWD 5' GACACTGACAGAGCACCTGA 3'

REV 5' CCTGGTGACAGCTTTGGAGA 3'

Fwd Tm 61.1°C

Rev Tm 61.6°C

GC 55%

Product 114 bp

Spans 2 introns

NM_014423 · chr5

116 Specific

FWD 5' AGTACTTTGCCTGCGATGCG 3'

REV 5' CAGGCGATGGTGCTTGAGAA 3'

Fwd Tm 62.9°C

Rev Tm 62.4°C

GC 55%

Product 137 bp

Spans 2 introns