AgmNAT 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_132040 · chrX

79 Single Exon Specific

FWD 5' CCAGACCTTCGTGCTCCATT 3'

REV 5' CCGGGCTGTCTTATCGTGTT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 139 bp

Single-exon gene (no intron)

NM_132040 · chrX

79 Single Exon Specific

FWD 5' CTTCCTGCTGGCCCACTATT 3'

REV 5' CACGAAGCATGTGCCAAAGG 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 121 bp

Single-exon gene (no intron)

NM_132040 · chrX

79 Single Exon Specific

FWD 5' CTTCCTGCTGGCCCACTATT 3'

REV 5' CCACGAAGCATGTGCCAAAG 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 122 bp

Single-exon gene (no intron)

NM_132040 · chrX

79 Single Exon Specific

FWD 5' ACCAAAGGACTCCCACGAAC 3'

REV 5' GGCGGATAGCAGATGCAGAA 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 94 bp

Single-exon gene (no intron)

NM_132040 · chrX

79 Single Exon Specific

FWD 5' CAGCTCATCAACACGTTGCG 3'

REV 5' AATGGAGCACGAAGGTCTGG 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 106 bp

Single-exon gene (no intron)

NM_132040 · chrX

79 Single Exon Specific

FWD 5' TGGGCTACCAGCTCATCAAC 3'

REV 5' AATGGAGCACGAAGGTCTGG 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 114 bp

Single-exon gene (no intron)

NM_132040 · chrX

79 Single Exon Specific

FWD 5' GGGCTACCAGCTCATCAACA 3'

REV 5' AATGGAGCACGAAGGTCTGG 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 113 bp

Single-exon gene (no intron)

NM_132040 · chrX

78 Single Exon Specific

FWD 5' TCCGTCGACTGTACCAGTGT 3'

REV 5' AGGTCTGGACACTCTCGTGT 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 148 bp

Single-exon gene (no intron)

NM_132040 · chrX

78 Single Exon Specific

FWD 5' CCTTTGGCACATGCTTCGTG 3'

REV 5' CCACTTACCGCCGGCATATT 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 128 bp

Single-exon gene (no intron)

NM_132040 · chrX

78 Single Exon Specific

FWD 5' CTTTGGCACATGCTTCGTGG 3'

REV 5' CCACTTACCGCCGGCATATT 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 127 bp

Single-exon gene (no intron)