Akap12 30 primer pairs found

Human

Show all (including non-specific)

NM_005100 · chr6

108 Specific

FWD 5' GCCACCAAGCTCCTACAGAA 3'

REV 5' GGCTGTTTAGGGCTCCTTGA 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 136 bp

Spans 1 intron

NM_005100 · chr6

108 Specific

FWD 5' GCCACCAAGCTCCTACAGAA 3'

REV 5' GTTTAGGGCTCCTTGACCGT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 132 bp

Spans 1 intron

NM_005100 · chr6

108 Specific

FWD 5' GCCACCAAGCTCCTACAGAA 3'

REV 5' CTGGCTGTTTAGGGCTCCTT 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 138 bp

Spans 1 intron

NM_005100 · chr6

108 Specific

FWD 5' GCCACCAAGCTCCTACAGAA 3'

REV 5' TGTTTAGGGCTCCTTGACCG 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 133 bp

Spans 1 intron

NM_005100 · chr6

108 Specific

FWD 5' GCCACCAAGCTCCTACAGAA 3'

REV 5' TGGCTGTTTAGGGCTCCTTG 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 137 bp

Spans 1 intron

NM_144497 · chr6

108 Specific

FWD 5' ACCCGCTAAGCTGATCTCCT 3'

REV 5' GTCGTGAACAACCGCTGACT 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 147 bp

Spans 1 intron

NM_005100 · chr6

108 Specific

FWD 5' GCCACCAAGCTCCTACAGAA 3'

REV 5' CTGGCCATTTAGGTCACCCT 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 99 bp

Spans 1 intron

NM_005100 · chr6

108 Specific

FWD 5' CAAGGAGCCCTAAACAGCCA 3'

REV 5' GTCGTGAACAACCGCTGACT 3'

Fwd Tm 62.0°C

Rev Tm 62.4°C

GC 55%

Product 129 bp

Spans 1 intron

NM_005100 · chr6

108 Specific

FWD 5' GCCACCAAGCTCCTACAGAA 3'

REV 5' TGGCCATTTAGGTCACCCTC 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 98 bp

Spans 1 intron

NM_005100 · chr6

108 Specific

FWD 5' GCCACCAAGCTCCTACAGAA 3'

REV 5' TCTGGCCATTTAGGTCACCC 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 100 bp

Spans 1 intron

NM_005100 · chr6

108 Specific

FWD 5' CGGTCAAGGAGCCCTAAACA 3'

REV 5' GTGATGTCGTGAACAACCGC 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 138 bp

Spans 1 intron

NM_144497 · chr6

108 Specific

FWD 5' GGGACCATCACCATCACAGT 3'

REV 5' GTGATGTCGTGAACAACCGC 3'

Fwd Tm 61.4°C

Rev Tm 61.5°C

GC 55%

Product 104 bp

Spans 1 intron

NM_144497 · chr6

107 Specific

FWD 5' ACCCGCTAAGCTGATCTCCT 3'

REV 5' TGTCGTGAACAACCGCTGAC 3'

Fwd Tm 62.5°C

Rev Tm 62.6°C

GC 55%

Product 148 bp

Spans 1 intron

NM_144497 · chr6

107 Specific

FWD 5' GGGACCATCACCATCACAGT 3'

REV 5' TCTCCTCCTGCCCATCATCT 3'

Fwd Tm 61.4°C

Rev Tm 62.0°C

GC 55%

Product 124 bp

Spans 1 intron

NM_144497 · chr6

107 Specific

FWD 5' GGGACCATCACCATCACAGT 3'

REV 5' GATGTCGTGAACAACCGCTG 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 55%

Product 102 bp

Spans 1 intron

NM_001370346 · chr6

107 Specific

FWD 5' CCCGAGATGGTTGGACAGAG 3'

REV 5' TCTCCTCCTGCCCATCATCT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 60%

Product 115 bp

Spans 1 intron

NM_144497 · chr6

107 Specific

FWD 5' GGGACCATCACCATCACAGT 3'

REV 5' CTCCTGCCCATCATCTGTGA 3'

Fwd Tm 61.4°C

Rev Tm 61.2°C

GC 55%

Product 120 bp

Spans 1 intron

NM_001370346 · chr6

106 Specific

FWD 5' CCAACTGTCCCTTAGGACCG 3'

REV 5' TCTCCTCCTGCCCATCATCT 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 60%

Product 136 bp

Spans 1 intron

NM_001370346 · chr6

106 Specific

FWD 5' CAACTGTCCCTTAGGACCGG 3'

REV 5' TCTCCTCCTGCCCATCATCT 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 60%

Product 135 bp

Spans 1 intron

NM_144497 · chr6

106 Specific

FWD 5' CCGCTAAGCTGATCTCCTGT 3'

REV 5' GATGTCGTGAACAACCGCTG 3'

Fwd Tm 61.1°C

Rev Tm 61.2°C

GC 55%

Product 148 bp

Spans 1 intron

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