Alg1 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_142405 · chr3R

109 Specific

FWD 5' AAGGTGGTCGACATGTTCGG 3'

REV 5' CGCCAAACACGAAGCCATTC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 103 bp

Spans 1 intron

NM_142405 · chr3R

109 Specific

FWD 5' TAGGCGACTGGAGCGATACT 3'

REV 5' GGGCGCACGATCATACAGAA 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 1 intron

NM_142405 · chr3R

109 Specific

FWD 5' CCTGTCTGCGCCTACGATTT 3'

REV 5' ACCAAATCCTCAGCTGCTCG 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 109 bp

Spans 1 intron

NM_142405 · chr3R

109 Specific

FWD 5' AAGGTGGTCGACATGTTCGG 3'

REV 5' GCTGAACATGGTCGCCAAAC 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 115 bp

Spans 1 intron

NM_142405 · chr3R

109 Specific

FWD 5' AAGGTGGTCGACATGTTCGG 3'

REV 5' ACCAAATCCTCAGCTGCTCG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 139 bp

Spans 1 intron

NM_142405 · chr3R

109 Specific

FWD 5' ATTGGTTAGGCGACTGGAGC 3'

REV 5' GGGCGCACGATCATACAGAA 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 133 bp

Spans 1 intron

NM_142405 · chr3R

109 Specific

FWD 5' ATACTTCGGCTCCAAGGCAC 3'

REV 5' GGGCGCACGATCATACAGAA 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 112 bp

Spans 1 intron

NM_142405 · chr3R

109 Specific

FWD 5' AAGGTGGTCGACATGTTCGG 3'

REV 5' GAACATGGTCGCCAAACACG 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 112 bp

Spans 1 intron

NM_142405 · chr3R

109 Specific

FWD 5' CTCCAAGGCACACACACACT 3'

REV 5' CAGCTCGTGCTTGTGAGTCA 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 145 bp

Spans 1 intron

NM_142405 · chr3R

109 Specific

FWD 5' CTCCAAGGCACACACACACT 3'

REV 5' ACAGCTCGTGCTTGTGAGTC 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 146 bp

Spans 1 intron