Alp4 20 primer pairs found

Drosophila

Show all (including non-specific)

NM_170534 · chr3R

110 Specific

FWD 5' TAGACGCGGAATTCTGGCAC 3'

REV 5' CGTGGATATTCCCATGCCGT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 143 bp

Spans 1 intron

NM_170534 · chr3R

109 1 gene · 2 transcripts

FWD 5' GGCAGTACCTGAAGCATGGT 3'

REV 5' AGAAGATCGCAGTGGCAGTG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 135 bp

Spans 1 intron

NM_079862 · chr3R

109 1 gene · 2 transcripts

FWD 5' GGCAGTACCTGAAGCATGGT 3'

REV 5' AGAAGATCGCAGTGGCAGTG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 135 bp

Spans 1 intron

NM_170534 · chr3R

109 Specific

FWD 5' TAAGCAGTGAACGGACGACG 3'

REV 5' AATGGTCTTCTCCAGCTGCC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 140 bp

Spans 1 intron

NM_170534 · chr3R

109 1 gene · 2 transcripts

FWD 5' GTATGCCAATGGTCCTGGCT 3'

REV 5' AAGTGGCGATAAGTGGGAGC 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 129 bp

Spans 1 intron

NM_079862 · chr3R

109 1 gene · 2 transcripts

FWD 5' GTATGCCAATGGTCCTGGCT 3'

REV 5' AAGTGGCGATAAGTGGGAGC 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 129 bp

Spans 1 intron

NM_170534 · chr3R

109 1 gene · 2 transcripts

FWD 5' GGCAGTACCTGAAGCATGGT 3'

REV 5' GAGAAGATCGCAGTGGCAGT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 136 bp

Spans 1 intron

NM_079862 · chr3R

109 1 gene · 2 transcripts

FWD 5' GGCAGTACCTGAAGCATGGT 3'

REV 5' GAGAAGATCGCAGTGGCAGT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 136 bp

Spans 1 intron

NM_170534 · chr3R

109 1 gene · 2 transcripts

FWD 5' GGCAGTACCTGAAGCATGGT 3'

REV 5' AACCCGAGAAGATCGCAGTG 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 1 intron

NM_079862 · chr3R

109 1 gene · 2 transcripts

FWD 5' GGCAGTACCTGAAGCATGGT 3'

REV 5' AACCCGAGAAGATCGCAGTG 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 1 intron

NM_170534 · chr3R

109 1 gene · 2 transcripts

FWD 5' GGCAGTACCTGAAGCATGGT 3'

REV 5' TTCGAACCCGAGAAGATCGC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 145 bp

Spans 1 intron

NM_079862 · chr3R

109 1 gene · 2 transcripts

FWD 5' GGCAGTACCTGAAGCATGGT 3'

REV 5' TTCGAACCCGAGAAGATCGC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 145 bp

Spans 1 intron

NM_170534 · chr3R

109 1 gene · 2 transcripts

FWD 5' TCGTATGCCAATGGTCCTGG 3'

REV 5' AAGTGGCGATAAGTGGGAGC 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 131 bp

Spans 1 intron

NM_079862 · chr3R

109 1 gene · 2 transcripts

FWD 5' TCGTATGCCAATGGTCCTGG 3'

REV 5' AAGTGGCGATAAGTGGGAGC 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 131 bp

Spans 1 intron

NM_170534 · chr3R

109 1 gene · 2 transcripts

FWD 5' TCGTATGCCAATGGTCCTGG 3'

REV 5' CCAAGTGGCGATAAGTGGGA 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 133 bp

Spans 1 intron

NM_079862 · chr3R

109 1 gene · 2 transcripts

FWD 5' TCGTATGCCAATGGTCCTGG 3'

REV 5' CCAAGTGGCGATAAGTGGGA 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 133 bp

Spans 1 intron

NM_170534 · chr3R

109 Specific

FWD 5' TAGACGCGGAATTCTGGCAC 3'

REV 5' TCGTGGATATTCCCATGCCG 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 144 bp

Spans 1 intron

NM_079862 · chr3R

108 1 gene · 2 transcripts

FWD 5' ATCCACGATCAGTGCTGGTC 3'

REV 5' CCGAATCCGGCACTTGTTTG 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 143 bp

Spans 1 intron

NM_079862 · chr3R

108 1 gene · 2 transcripts

FWD 5' CTGAAGCATGGTTACGGCGA 3'

REV 5' AGAAGATCGCAGTGGCAGTG 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 127 bp

Spans 1 intron

NM_079862 · chr3R

108 1 gene · 2 transcripts

FWD 5' TGAAGCATGGTTACGGCGAG 3'

REV 5' AGAAGATCGCAGTGGCAGTG 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 126 bp

Spans 1 intron