Ama 30 primer pairs found

Drosophila

Show all (including non-specific)

NM_176412 · chr3R

109 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' ACCGATTAGAAGCCGCAGAC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 109 bp

Spans 1 intron

NM_057231 · chr3R

109 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' ACCGATTAGAAGCCGCAGAC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 125 bp

Spans 1 intron

NM_176412 · chr3R

108 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' AGACCGATTAGAAGCCGCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 111 bp

Spans 1 intron

NM_057231 · chr3R

108 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' AGACCGATTAGAAGCCGCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 127 bp

Spans 1 intron

NM_176412 · chr3R

108 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' GACCGATTAGAAGCCGCAGA 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 110 bp

Spans 1 intron

NM_057231 · chr3R

108 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' GACCGATTAGAAGCCGCAGA 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 126 bp

Spans 1 intron

NM_176412 · chr3R

107 Specific

FWD 5' ACATCCTACCAGACATGGCG 3'

REV 5' GTCCTTGCTGATCTGGCTGA 3'

Fwd Tm 61.4°C

Rev Tm 61.6°C

GC 55%

Product 113 bp

Spans 1 intron

NM_176412 · chr3R

107 Specific

FWD 5' ACATCCTACCAGACATGGCG 3'

REV 5' ACCACGTCCTTGCTGATCTG 3'

Fwd Tm 61.4°C

Rev Tm 61.8°C

GC 55%

Product 118 bp

Spans 1 intron

NM_176412 · chr3R

107 Specific

FWD 5' ACATCCTACCAGACATGGCG 3'

REV 5' CACCACGTCCTTGCTGATCT 3'

Fwd Tm 61.4°C

Rev Tm 61.8°C

GC 55%

Product 119 bp

Spans 1 intron

NM_176412 · chr3R

107 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' CCGAATCCAAGCTGATCGCA 3'

Fwd Tm 61.9°C

Rev Tm 62.4°C

GC 55%

Product 143 bp

Spans 1 intron

NM_176412 · chr3R

107 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' ACCGAATCCAAGCTGATCGC 3'

Fwd Tm 61.9°C

Rev Tm 62.5°C

GC 55%

Product 144 bp

Spans 1 intron

NM_176412 · chr3R

107 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' AAGCTGATCGCAAGGCAGAA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 135 bp

Spans 1 intron

NM_057231 · chr3R

107 Specific

FWD 5' AATACCAGACATGGCGCGTC 3'

REV 5' ACCACGTCCTTGCTGATCTG 3'

Fwd Tm 62.4°C

Rev Tm 61.8°C

GC 55%

Product 114 bp

Spans 1 intron

NM_057231 · chr3R

107 Specific

FWD 5' AATACCAGACATGGCGCGTC 3'

REV 5' CACCACGTCCTTGCTGATCT 3'

Fwd Tm 62.4°C

Rev Tm 61.8°C

GC 55%

Product 115 bp

Spans 1 intron

NM_057231 · chr3R

107 Specific

FWD 5' AAATACCAGACATGGCGCGT 3'

REV 5' ACCACGTCCTTGCTGATCTG 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 50%

Product 115 bp

Spans 1 intron

NM_057231 · chr3R

107 Specific

FWD 5' AAATACCAGACATGGCGCGT 3'

REV 5' CACCACGTCCTTGCTGATCT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 50%

Product 116 bp

Spans 1 intron

NM_057231 · chr3R

107 Specific

FWD 5' AATACCAGACATGGCGCGTC 3'

REV 5' TTGAACTCCACAGAGTCGCC 3'

Fwd Tm 62.4°C

Rev Tm 61.7°C

GC 55%

Product 144 bp

Spans 1 intron

NM_176412 · chr3R

106 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' AGCTGATCGCAAGGCAGAAT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 134 bp

Spans 1 intron

NM_057231 · chr3R

106 1 gene · 2 transcripts

FWD 5' GTGAAGCGCATTGAACCCAC 3'

REV 5' AGCTGATCGCAAGGCAGAAT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 150 bp

Spans 1 intron

NM_057231 · chr3R

106 Specific

FWD 5' GAAATACCAGACATGGCGCG 3'

REV 5' GTCCTTGCTGATCTGGCTGA 3'

Fwd Tm 61.2°C

Rev Tm 61.6°C

GC 55%

Product 111 bp

Spans 1 intron

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