Amt 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_001104330 · chr3R

110 Specific

FWD 5' CTAAGTGGCAGTACGCAGCA 3'

REV 5' AGCCGAGCACTCCATTGATC 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001104330 · chr3R

110 Specific

FWD 5' CTAAGTGGCAGTACGCAGCA 3'

REV 5' GAGCCGAGCACTCCATTGAT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001104330 · chr3R

110 Specific

FWD 5' GGGATCATTTGGAGGCGGAT 3'

REV 5' AGCCGAGCACTCCATTGATC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001104330 · chr3R

110 Specific

FWD 5' GGGATCATTTGGAGGCGGAT 3'

REV 5' GAGCCGAGCACTCCATTGAT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001104330 · chr3R

109 Specific

FWD 5' TTTGGAGGCGGATTCACCAG 3'

REV 5' GCCGAGCACTCCATTGATCA 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 90 bp

Spans 1 intron

NM_001104330 · chr3R

109 Specific

FWD 5' ATGGGATCATTTGGAGGCGG 3'

REV 5' GCCGAGCACTCCATTGATCA 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001104330 · chr3R

109 Specific

FWD 5' CTAAGTGGCAGTACGCAGCA 3'

REV 5' GCCGAGCACTCCATTGATCA 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 55%

Product 140 bp

Spans 1 intron

NM_001104330 · chr3R

109 Specific

FWD 5' ATGGGATCATTTGGAGGCGG 3'

REV 5' AGCCGAGCACTCCATTGATC 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001104330 · chr3R

109 Specific

FWD 5' ATGGGATCATTTGGAGGCGG 3'

REV 5' GAGCCGAGCACTCCATTGAT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 101 bp

Spans 1 intron

NM_001104330 · chr3R

109 Specific

FWD 5' TAAGTGGCAGTACGCAGCAC 3'

REV 5' GCCGAGCACTCCATTGATCA 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 139 bp

Spans 1 intron