Ance-5 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_133164 · chr2R

108 Specific

FWD 5' GTTCAGCCGCCATTACAACG 3'

REV 5' CAGGATCTCGGCCAGGAATT 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 111 bp

Spans 1 intron

NM_133164 · chr2R

108 Specific

FWD 5' CAAATTCGCCGGAGTTCAGC 3'

REV 5' CAGGATCTCGGCCAGGAATT 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 124 bp

Spans 1 intron

NM_133164 · chr2R

107 Specific

FWD 5' GGAGTTCAGCCGCCATTACA 3'

REV 5' CAGGATCTCGGCCAGGAATT 3'

Fwd Tm 62.2°C

Rev Tm 61.5°C

GC 55%

Product 114 bp

Spans 1 intron

NM_133164 · chr2R

106 Specific

FWD 5' AAATCAACCGCAAAGCTGCC 3'

REV 5' TACCTGCGACTTGCACTCAG 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 50%

Product 128 bp

Spans 1 intron

NM_133164 · chr2R

106 Specific

FWD 5' GGACAAATTCGCCGGAGTTC 3'

REV 5' CAGGATCTCGGCCAGGAATT 3'

Fwd Tm 61.1°C

Rev Tm 61.5°C

GC 55%

Product 127 bp

Spans 1 intron

NM_133164 · chr2R

106 Specific

FWD 5' CAGGACAAATTCGCCGGAGT 3'

REV 5' CAGGATCTCGGCCAGGAATT 3'

Fwd Tm 62.4°C

Rev Tm 61.5°C

GC 55%

Product 129 bp

Spans 1 intron

NM_133164 · chr2R

105 Specific

FWD 5' AGGACAAATTCGCCGGAGTT 3'

REV 5' CAGGATCTCGGCCAGGAATT 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 50%

Product 128 bp

Spans 1 intron

NM_133164 · chr2R

105 Specific

FWD 5' GTTCAGCCGCCATTACAACG 3'

REV 5' CTGGAATCCCAGGATCTCGG 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 120 bp

Spans 1 intron

NM_133164 · chr2R

105 Specific

FWD 5' CAAATTCGCCGGAGTTCAGC 3'

REV 5' CTGGAATCCCAGGATCTCGG 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 133 bp

Spans 1 intron

NM_133164 · chr2R

105 Specific

FWD 5' AGCCGCCATTACAACGTCTT 3'

REV 5' CAGGATCTCGGCCAGGAATT 3'

Fwd Tm 61.9°C

Rev Tm 61.5°C

GC 50%

Product 107 bp

Spans 1 intron