NM_001274428 · chr3L

109 Specific

FWD 5' ATCGAACTTAAGGCCCACCG 3'

REV 5' ACCCAAGGTCGTCCAAACTC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 134 bp

Spans 1 intron

NCBI BLAST

NM_001274428 · chr3L

109 Specific

FWD 5' AGCTGTGTACGTATGCAGCG 3'

REV 5' GTTGGATGAATACGGCCGGT 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 95 bp

Spans 1 intron

NCBI BLAST

NM_001274428 · chr3L

109 Specific

FWD 5' AGCTGTGTACGTATGCAGCG 3'

REV 5' TTGGATGAATACGGCCGGTG 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 94 bp

Spans 1 intron

NCBI BLAST

NM_001274428 · chr3L

109 Specific

FWD 5' AGCTGTGTACGTATGCAGCG 3'

REV 5' TCGTTGGATGAATACGGCCG 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 97 bp

Spans 1 intron

NCBI BLAST

NM_001274428 · chr3L

109 Specific

FWD 5' ATCGAACTTAAGGCCCACCG 3'

REV 5' CGTACCCAAGGTCGTCCAAA 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 137 bp

Spans 1 intron

NCBI BLAST

NM_001274428 · chr3L

109 Specific

FWD 5' ATCGAACTTAAGGCCCACCG 3'

REV 5' CCAAGGTCGTCCAAACTCGA 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 132 bp

Spans 1 intron

NCBI BLAST

NM_001274428 · chr3L

108 Specific

FWD 5' CGGCCGTATTCATCCAACGA 3'

REV 5' ACCCAAGGTCGTCCAAACTC 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 116 bp

Spans 1 intron

NCBI BLAST

NM_001274428 · chr3L

108 Specific

FWD 5' CGGCCGTATTCATCCAACGA 3'

REV 5' CGTACCCAAGGTCGTCCAAA 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 119 bp

Spans 1 intron

NCBI BLAST

NM_001274428 · chr3L

108 Specific

FWD 5' CACCGGCCGTATTCATCCAA 3'

REV 5' ACCCAAGGTCGTCCAAACTC 3'

Fwd Tm 62.2°C

Rev Tm 61.8°C

GC 55%

Product 119 bp

Spans 1 intron

NCBI BLAST

NM_001274428 · chr3L

108 Specific

FWD 5' CGGCCGTATTCATCCAACGA 3'

REV 5' CCAAGGTCGTCCAAACTCGA 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 114 bp

Spans 1 intron

NCBI BLAST

NM_139519 · chr3L

80 Single Exon 1 gene · 2 transcripts

FWD 5' CCCAAACTCACTGGCCAGAT 3'

REV 5' GTTTGCGTGCAAGTGGACTG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 109 bp

Single-exon gene (no intron)

NCBI BLAST

NM_139519 · chr3L

80 Single Exon 1 gene · 2 transcripts

FWD 5' CACGCCGGATATTCGGGAAT 3'

REV 5' AATTGGCCCGATCACAGAGG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 148 bp

Single-exon gene (no intron)

NCBI BLAST

NM_139519 · chr3L

80 Single Exon 1 gene · 2 transcripts

FWD 5' CTGTCCCACATCCAAACCCA 3'

REV 5' GTTTGCGTGCAAGTGGACTG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 125 bp

Single-exon gene (no intron)

NCBI BLAST

NM_139519 · chr3L

80 Single Exon 1 gene · 2 transcripts

FWD 5' TTTACCGGCTGCAGAGGAAC 3'

REV 5' TCAGATCGTCCTCCTCCGTT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 119 bp

Single-exon gene (no intron)

NCBI BLAST

NM_139519 · chr3L

80 Single Exon 1 gene · 2 transcripts

FWD 5' TTTACCGGCTGCAGAGGAAC 3'

REV 5' TTCAGATCGTCCTCCTCCGT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 120 bp

Single-exon gene (no intron)

NCBI BLAST

NM_139519 · chr3L

79 Single Exon 1 gene · 2 transcripts

FWD 5' CCTCTGTGATCGGGCCAATT 3'

REV 5' TCCAGGTGGCTCTTCTGGTA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 133 bp

Single-exon gene (no intron)

NCBI BLAST

NM_139519 · chr3L

79 Single Exon 1 gene · 2 transcripts

FWD 5' TACCAGAAGAGCCACCTGGA 3'

REV 5' CCAGCCTTGCTGTTGTAGGT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 140 bp

Single-exon gene (no intron)

NCBI BLAST

NM_139519 · chr3L

79 Single Exon 1 gene · 2 transcripts

FWD 5' CACGCCGGATATTCGGGAAT 3'

REV 5' GCAGGTGCAGCTGATAGTGA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 101 bp

Single-exon gene (no intron)

NCBI BLAST

NM_139519 · chr3L

79 Single Exon 1 gene · 2 transcripts

FWD 5' TTTACCGGCTGCAGAGGAAC 3'

REV 5' CGTCCTCCTCCGTTTGTGTT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 113 bp

Single-exon gene (no intron)

NCBI BLAST

NM_139519 · chr3L

79 Single Exon 1 gene · 2 transcripts

FWD 5' CTGCAGCACCATCTGGAGAA 3'

REV 5' GGGAACCTTGTCGGATTCGT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Single-exon gene (no intron)

NCBI BLAST

Asciz 20 primer pairs found