Ask1 20 primer pairs found

Drosophila

Show all (including non-specific)

NM_057741 · chr3R

110 Specific

FWD 5' TTCCAAGCAGACGCTCAGTC 3'

REV 5' CAGGTGGCAGGAATTGGTCT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 91 bp

Spans 1 intron

NM_057741 · chr3R

110 Specific

FWD 5' TTCCAAGCAGACGCTCAGTC 3'

REV 5' CTGGTCAGGTGGCAGGAATT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 96 bp

Spans 1 intron

NM_206518 · chr3R

110 1 gene · 2 transcripts

FWD 5' TTCAGCGGGTTCAGTTCGAG 3'

REV 5' TCTCCCTAACGCCGTAGTGA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 1 intron

NM_057741 · chr3R

110 1 gene · 2 transcripts

FWD 5' TTCAGCGGGTTCAGTTCGAG 3'

REV 5' TCTCCCTAACGCCGTAGTGA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 1 intron

NM_206518 · chr3R

110 1 gene · 2 transcripts

FWD 5' AAAGCTGGACCTCATGAGGC 3'

REV 5' ACTGTCCACACAATCGGACG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 106 bp

Spans 1 intron

NM_057741 · chr3R

110 Specific

FWD 5' TTCCAAGCAGACGCTCAGTC 3'

REV 5' TCAGGTGGCAGGAATTGGTC 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 92 bp

Spans 1 intron

NM_057741 · chr3R

110 1 gene · 2 transcripts

FWD 5' AAAGCTGGACCTCATGAGGC 3'

REV 5' ACTGTCCACACAATCGGACG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 106 bp

Spans 1 intron

NM_057741 · chr3R

110 Specific

FWD 5' TCGATTCCAAGCAGACGCTC 3'

REV 5' CAGGTGGCAGGAATTGGTCT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 1 intron

NM_057741 · chr3R

110 Specific

FWD 5' TCGATTCCAAGCAGACGCTC 3'

REV 5' CTGGTCAGGTGGCAGGAATT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 100 bp

Spans 1 intron

NM_206518 · chr3R

110 1 gene · 2 transcripts

FWD 5' TACATGGGCGGCAGAAAGAG 3'

REV 5' TTGCCCGCTATATTCGGACG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 116 bp

Spans 1 intron

NM_057741 · chr3R

110 1 gene · 2 transcripts

FWD 5' TACATGGGCGGCAGAAAGAG 3'

REV 5' TTGCCCGCTATATTCGGACG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 116 bp

Spans 1 intron

NM_206518 · chr3R

110 1 gene · 2 transcripts

FWD 5' GTCAGGAGCTGGCTCACATT 3'

REV 5' TTGCCCGCTATATTCGGACG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 136 bp

Spans 1 intron

NM_057741 · chr3R

110 1 gene · 2 transcripts

FWD 5' GTCAGGAGCTGGCTCACATT 3'

REV 5' TTGCCCGCTATATTCGGACG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 136 bp

Spans 1 intron

NM_057741 · chr3R

110 Specific

FWD 5' TCGATTCCAAGCAGACGCTC 3'

REV 5' GTTCCTTGCTGTTGTTGCCG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 132 bp

Spans 1 intron

NM_206518 · chr3R

110 1 gene · 2 transcripts

FWD 5' TTCAGCGGGTTCAGTTCGAG 3'

REV 5' TGCTGGGTGAGAATGCTCAG 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 106 bp

Spans 1 intron

NM_206518 · chr3R

110 1 gene · 2 transcripts

FWD 5' TTCAGCGGGTTCAGTTCGAG 3'

REV 5' CTGCTGGGTGAGAATGCTCA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 107 bp

Spans 1 intron

NM_206518 · chr3R

110 1 gene · 2 transcripts

FWD 5' GGCAACAACAGCAAGGAACC 3'

REV 5' CCGTTCGCATGTCACTCAGA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 1 intron

NM_206518 · chr3R

110 1 gene · 2 transcripts

FWD 5' GCAACAACAGCAAGGAACCC 3'

REV 5' CCGTTCGCATGTCACTCAGA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 1 intron

NM_206518 · chr3R

110 1 gene · 2 transcripts

FWD 5' GAAGGACGAGTGCCCACTTT 3'

REV 5' CCGTTCGCATGTCACTCAGA 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 109 bp

Spans 1 intron

NM_206518 · chr3R

110 1 gene · 2 transcripts

FWD 5' AAAGCTGGACCTCATGAGGC 3'

REV 5' AAGTGCTTGGTGGACTGTCC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 119 bp

Spans 1 intron