Atg12 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_140294 · chr3L

109 Specific

FWD 5' CAACAAGACAGTCGGCTGGA 3'

REV 5' GGTTCCATGGCACTCGTACA 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 145 bp

Spans 1 intron

NM_140294 · chr3L

109 Specific

FWD 5' CAACAAGACAGTCGGCTGGA 3'

REV 5' TGGTTCCATGGCACTCGTAC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 146 bp

Spans 1 intron

NM_140294 · chr3L

109 Specific

FWD 5' ATCCCAACAAGACAGTCGGC 3'

REV 5' GGTTCCATGGCACTCGTACA 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 149 bp

Spans 1 intron

NM_140294 · chr3L

109 Specific

FWD 5' ATCCCAACAAGACAGTCGGC 3'

REV 5' TGGTTCCATGGCACTCGTAC 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 150 bp

Spans 1 intron

NM_140294 · chr3L

108 Specific

FWD 5' GTCGGCTGGATACAGACGTT 3'

REV 5' GGTTCCATGGCACTCGTACA 3'

Fwd Tm 61.5°C

Rev Tm 61.8°C

GC 55%

Product 135 bp

Spans 1 intron

NM_140294 · chr3L

108 Specific

FWD 5' GTCGGCTGGATACAGACGTT 3'

REV 5' TGGTTCCATGGCACTCGTAC 3'

Fwd Tm 61.5°C

Rev Tm 61.8°C

GC 55%

Product 136 bp

Spans 1 intron

NM_140294 · chr3L

108 Specific

FWD 5' CGGCTGGATACAGACGTTCA 3'

REV 5' GGTTCCATGGCACTCGTACA 3'

Fwd Tm 61.5°C

Rev Tm 61.8°C

GC 55%

Product 133 bp

Spans 1 intron

NM_140294 · chr3L

108 Specific

FWD 5' CGGCTGGATACAGACGTTCA 3'

REV 5' TGGTTCCATGGCACTCGTAC 3'

Fwd Tm 61.5°C

Rev Tm 61.8°C

GC 55%

Product 134 bp

Spans 1 intron

NM_140294 · chr3L

108 Specific

FWD 5' TCTGAAACTCGATGCCAGCG 3'

REV 5' GGTTCCATGGCACTCGTACA 3'

Fwd Tm 62.3°C

Rev Tm 61.8°C

GC 55%

Product 103 bp

Spans 1 intron

NM_140294 · chr3L

108 Specific

FWD 5' TCTGAAACTCGATGCCAGCG 3'

REV 5' TGGTTCCATGGCACTCGTAC 3'

Fwd Tm 62.3°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 1 intron