Atg5 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_132162 · chrX

118 Specific

FWD 5' TGGTGACTGACAAGGTTCGC 3'

REV 5' CCGGATGCAGCAGATCGTAT 3'

Fwd Tm 62.2°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 2 introns

NM_132162 · chrX

118 Specific

FWD 5' GGTGACTGACAAGGTTCGCA 3'

REV 5' CCGGATGCAGCAGATCGTAT 3'

Fwd Tm 62.2°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_132162 · chrX

116 Specific

FWD 5' TGGTGACTGACAAGGTTCGC 3'

REV 5' TCCGGATGCAGCAGATCGTA 3'

Fwd Tm 62.2°C

Rev Tm 62.9°C

GC 55%

Product 142 bp

Spans 2 introns

NM_132162 · chrX

116 Specific

FWD 5' GGTGACTGACAAGGTTCGCA 3'

REV 5' TCCGGATGCAGCAGATCGTA 3'

Fwd Tm 62.2°C

Rev Tm 62.9°C

GC 55%

Product 141 bp

Spans 2 introns

NM_132162 · chrX

114 Specific

FWD 5' GCCTTTGGTGACTGACAAGG 3'

REV 5' CCGGATGCAGCAGATCGTAT 3'

Fwd Tm 60.8°C

Rev Tm 61.8°C

GC 55%

Product 146 bp

Spans 2 introns

NM_132162 · chrX

112 Specific

FWD 5' CGATGACTTCACGTACACCC 3'

REV 5' CCTCGTGTAGATCAATGCCG 3'

Fwd Tm 59.9°C

Rev Tm 60.1°C

GC 55%

Product 146 bp

Spans 2 introns

NM_132162 · chrX

111 Specific

FWD 5' CGATGACTTCACGTACACCC 3'

REV 5' GTAGATCAATGCCGTGCGTG 3'

Fwd Tm 59.9°C

Rev Tm 61.1°C

GC 55%

Product 140 bp

Spans 2 introns

NM_132162 · chrX

111 Specific

FWD 5' CGATGACTTCACGTACACCC 3'

REV 5' GTGTAGATCAATGCCGTGCG 3'

Fwd Tm 59.9°C

Rev Tm 61.1°C

GC 55%

Product 142 bp

Spans 2 introns

NM_132162 · chrX

111 Specific

FWD 5' CGATGACTTCACGTACACCC 3'

REV 5' CGTGTAGATCAATGCCGTGC 3'

Fwd Tm 59.9°C

Rev Tm 61.1°C

GC 55%

Product 143 bp

Spans 2 introns

NM_132162 · chrX

110 Specific

FWD 5' ATCGCGACTCAGCTACTTGC 3'

REV 5' AAGTCGAACCACACAGCTCC 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 102 bp

Spans 1 intron