BBS1 10 primer pairs found

Drosophila

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NM_139823 · chr3L

109 Specific

FWD 5' TCCATCTTTGTGGAGCAGGC 3'

REV 5' TATCGTGCTCTCCGACGAGT 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 141 bp

Spans 1 intron

NM_139823 · chr3L

109 Specific

FWD 5' TCCATCTTTGTGGAGCAGGC 3'

REV 5' CGACGAGTTGATGGCATCGA 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 129 bp

Spans 1 intron

NM_139823 · chr3L

109 Specific

FWD 5' TACATCGACGAAACGGAGCC 3'

REV 5' CTGTGCGGGAATCGTGTACT 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 111 bp

Spans 1 intron

NM_139823 · chr3L

109 Specific

FWD 5' TACATCGACGAAACGGAGCC 3'

REV 5' TTTCGCCATACCTCCAGCTC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 146 bp

Spans 1 intron

NM_139823 · chr3L

108 Specific

FWD 5' TCCATCTTTGTGGAGCAGGC 3'

REV 5' CTCCGACGAGTTGATGGCAT 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 132 bp

Spans 1 intron

NM_139823 · chr3L

108 Specific

FWD 5' TCCATCTTTGTGGAGCAGGC 3'

REV 5' TCCGACGAGTTGATGGCATC 3'

Fwd Tm 62.2°C

Rev Tm 61.8°C

GC 55%

Product 131 bp

Spans 1 intron

NM_139823 · chr3L

108 Specific

FWD 5' GCCCAAGAAGAGCTCCATCT 3'

REV 5' TGCTCTCCGACGAGTTGATG 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 149 bp

Spans 1 intron

NM_139823 · chr3L

108 Specific

FWD 5' GCCCAAGAAGAGCTCCATCT 3'

REV 5' GTGCTCTCCGACGAGTTGAT 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 150 bp

Spans 1 intron

NM_139823 · chr3L

108 Specific

FWD 5' AGCCCAAGAAGAGCTCCATC 3'

REV 5' TCCGACGAGTTGATGGCATC 3'

Fwd Tm 61.5°C

Rev Tm 61.8°C

GC 55%

Product 145 bp

Spans 1 intron

NM_139823 · chr3L

108 Specific

FWD 5' GCCCAAGAAGAGCTCCATCT 3'

REV 5' TCCGACGAGTTGATGGCATC 3'

Fwd Tm 61.5°C

Rev Tm 61.8°C

GC 55%

Product 144 bp

Spans 1 intron