BBS4 20 primer pairs found

Drosophila

Show all (including non-specific)

NM_136792 · chr2R

116 1 gene · 2 transcripts

FWD 5' CAGTCCCGCAACGATATCGA 3'

REV 5' TTTGCGCAGCGATGAAATGG 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 147 bp

Spans 2 introns

NM_001299376 · chr2R

116 1 gene · 2 transcripts

FWD 5' CAGTCCCGCAACGATATCGA 3'

REV 5' TTTGCGCAGCGATGAAATGG 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 147 bp

Spans 2 introns

NM_136792 · chr2R

115 1 gene · 2 transcripts

FWD 5' TGCAGTCCCGCAACGATATC 3'

REV 5' TTTGCGCAGCGATGAAATGG 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001299376 · chr2R

115 1 gene · 2 transcripts

FWD 5' TGCAGTCCCGCAACGATATC 3'

REV 5' TTTGCGCAGCGATGAAATGG 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 149 bp

Spans 2 introns

NM_136792 · chr2R

115 1 gene · 2 transcripts

FWD 5' CAGTCCCGCAACGATATCGA 3'

REV 5' GGATTTGCGCAGCGATGAAA 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001299376 · chr2R

115 1 gene · 2 transcripts

FWD 5' CAGTCCCGCAACGATATCGA 3'

REV 5' GGATTTGCGCAGCGATGAAA 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 150 bp

Spans 2 introns

NM_136792 · chr2R

114 1 gene · 2 transcripts

FWD 5' TTGCAGTCCCGCAACGATAT 3'

REV 5' TTTGCGCAGCGATGAAATGG 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 50%

Product 150 bp

Spans 2 introns

NM_001299376 · chr2R

114 1 gene · 2 transcripts

FWD 5' TTGCAGTCCCGCAACGATAT 3'

REV 5' TTTGCGCAGCGATGAAATGG 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 50%

Product 150 bp

Spans 2 introns

NM_136792 · chr2R

113 Specific

FWD 5' TGTGGCCATTTCATCGCTGC 3'

REV 5' CCTTGCGCAGGTTTATGGCT 3'

Fwd Tm 63.5°C

Rev Tm 63.1°C

GC 55%

Product 146 bp

Spans 2 introns

NM_136792 · chr2R

113 Specific

FWD 5' TGTGGCCATTTCATCGCTGC 3'

REV 5' TCCTTGCGCAGGTTTATGGC 3'

Fwd Tm 63.5°C

Rev Tm 63.0°C

GC 55%

Product 147 bp

Spans 2 introns

NM_136792 · chr2R

113 Specific

FWD 5' TTGTGGCCATTTCATCGCTG 3'

REV 5' GGCACTGGCGTATTGTTCAG 3'

Fwd Tm 61.0°C

Rev Tm 61.1°C

GC 50%

Product 110 bp

Spans 2 introns

NM_001299376 · chr2R

113 1 gene · 2 transcripts

FWD 5' TGTGGCCATTTCATCGCTGC 3'

REV 5' GCCACGAATGCGTTCTCCAT 3'

Fwd Tm 63.5°C

Rev Tm 63.0°C

GC 55%

Product 132 bp

Spans 2 introns

NM_136792 · chr2R

113 Specific

FWD 5' TGTGGCCATTTCATCGCTGC 3'

REV 5' GTGTGAAAGGCACTGGCGTA 3'

Fwd Tm 63.5°C

Rev Tm 62.5°C

GC 55%

Product 117 bp

Spans 2 introns

NM_136792 · chr2R

113 Specific

FWD 5' TGTGGCCATTTCATCGCTGC 3'

REV 5' TTGCGCAGGTTTATGGCTGC 3'

Fwd Tm 63.5°C

Rev Tm 63.8°C

GC 55%

Product 144 bp

Spans 2 introns

NM_136792 · chr2R

113 Specific

FWD 5' TTGTGGCCATTTCATCGCTG 3'

REV 5' CTTGCGCAGGTTTATGGCTG 3'

Fwd Tm 61.0°C

Rev Tm 61.7°C

GC 50%

Product 146 bp

Spans 2 introns

NM_001299376 · chr2R

112 1 gene · 2 transcripts

FWD 5' GCAGAAGTTCATTGTGGCCA 3'

REV 5' CCATATCGTCCAGCTTTCGC 3'

Fwd Tm 60.6°C

Rev Tm 60.7°C

GC 50%

Product 128 bp

Spans 2 introns

NM_001299376 · chr2R

112 1 gene · 2 transcripts

FWD 5' TTGTGGCCATTTCATCGCTG 3'

REV 5' CCATATCGTCCAGCTTTCGC 3'

Fwd Tm 61.0°C

Rev Tm 60.7°C

GC 50%

Product 117 bp

Spans 2 introns

NM_001299376 · chr2R

111 1 gene · 2 transcripts

FWD 5' TGTGGCCATTTCATCGCTGC 3'

REV 5' CCTTTCGAGGGCCACGAATG 3'

Fwd Tm 63.5°C

Rev Tm 63.2°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001299376 · chr2R

111 1 gene · 2 transcripts

FWD 5' AGCAGAAGTTCATTGTGGCC 3'

REV 5' CCATATCGTCCAGCTTTCGC 3'

Fwd Tm 60.4°C

Rev Tm 60.7°C

GC 50%

Product 129 bp

Spans 2 introns

NM_001299376 · chr2R

111 1 gene · 2 transcripts

FWD 5' TGTGGCCATTTCATCGCTGC 3'

REV 5' CTTTCGAGGGCCACGAATGC 3'

Fwd Tm 63.5°C

Rev Tm 63.7°C

GC 55%

Product 141 bp

Spans 2 introns