BEAF-32 30 primer pairs found

Drosophila

Show all (including non-specific)

NM_079023 · chr2R

109 Specific

FWD 5' CACTCCAGCAGCAGTAACGA 3'

REV 5' GCGACGTTCGACTCGATCTT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 101 bp

Spans 1 intron

NM_079023 · chr2R

109 Specific

FWD 5' ACTCCAGCAGCAGTAACGAC 3'

REV 5' GCGACGTTCGACTCGATCTT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 100 bp

Spans 1 intron

NM_079023 · chr2R

109 Specific

FWD 5' CTCCAGCAGCAGTAACGACA 3'

REV 5' GCGACGTTCGACTCGATCTT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 99 bp

Spans 1 intron

NM_079023 · chr2R

109 Specific

FWD 5' TCTCCACTCCAGCAGCAGTA 3'

REV 5' TCGCCCAGATAATTGGCGAC 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 120 bp

Spans 1 intron

NM_079023 · chr2R

109 Specific

FWD 5' CACTCCAGCAGCAGTAACGA 3'

REV 5' CTCGCCCAGATAATTGGCGA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 117 bp

Spans 1 intron

NM_079023 · chr2R

109 Specific

FWD 5' ACTCCAGCAGCAGTAACGAC 3'

REV 5' CTCGCCCAGATAATTGGCGA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 116 bp

Spans 1 intron

NM_079023 · chr2R

109 Specific

FWD 5' CTCCAGCAGCAGTAACGACA 3'

REV 5' CTCGCCCAGATAATTGGCGA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 115 bp

Spans 1 intron

NM_079023 · chr2R

109 Specific

FWD 5' AAACTGGAACCGGATCCCTG 3'

REV 5' GCGACGTTCGACTCGATCTT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 139 bp

Spans 1 intron

NM_079023 · chr2R

108 Specific

FWD 5' TCTCCACTCCAGCAGCAGTA 3'

REV 5' CTCGCCCAGATAATTGGCGA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 121 bp

Spans 1 intron

NM_079023 · chr2R

108 Specific

FWD 5' TCTCCACTCCAGCAGCAGTA 3'

REV 5' GCCCAGATAATTGGCGACGT 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 55%

Product 118 bp

Spans 1 intron

NM_166069 · chr2R

106 1 gene · 2 transcripts

FWD 5' ACGACCAATTTGTGGACGCA 3'

REV 5' TCTTGGCCTTGGTAATCCGC 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 50%

Product 136 bp

Spans 1 intron

NM_001259399 · chr2R

106 1 gene · 2 transcripts

FWD 5' ACGACCAATTTGTGGACGCA 3'

REV 5' TCTTGGCCTTGGTAATCCGC 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 50%

Product 136 bp

Spans 1 intron

NM_166069 · chr2R

106 1 gene · 2 transcripts

FWD 5' ACGACCAATTTGTGGACGCA 3'

REV 5' CAGCGTCTTGGCCTTGGTAA 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 50%

Product 141 bp

Spans 1 intron

NM_001259399 · chr2R

106 1 gene · 2 transcripts

FWD 5' ACGACCAATTTGTGGACGCA 3'

REV 5' CAGCGTCTTGGCCTTGGTAA 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 50%

Product 141 bp

Spans 1 intron

NM_166069 · chr2R

106 1 gene · 2 transcripts

FWD 5' ACGACCAATTTGTGGACGCA 3'

REV 5' CCTTGGTAATCCGCACGGTT 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 50%

Product 130 bp

Spans 1 intron

NM_001259399 · chr2R

106 1 gene · 2 transcripts

FWD 5' ACGACCAATTTGTGGACGCA 3'

REV 5' CCTTGGTAATCCGCACGGTT 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 50%

Product 130 bp

Spans 1 intron

NM_166069 · chr2R

105 1 gene · 2 transcripts

FWD 5' ACGACCAATTTGTGGACGCA 3'

REV 5' CTTGGCCTTGGTAATCCGCA 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 50%

Product 135 bp

Spans 1 intron

NM_001259399 · chr2R

105 1 gene · 2 transcripts

FWD 5' ACGACCAATTTGTGGACGCA 3'

REV 5' CTTGGCCTTGGTAATCCGCA 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 50%

Product 135 bp

Spans 1 intron

NM_166069 · chr2R

105 1 gene · 2 transcripts

FWD 5' ATTTGTGGACGCATCTGCGG 3'

REV 5' TTGGCCTTGGTAATCCGCAC 3'

Fwd Tm 63.2°C

Rev Tm 62.8°C

GC 55%

Product 127 bp

Spans 1 intron

NM_001259399 · chr2R

105 1 gene · 2 transcripts

FWD 5' ATTTGTGGACGCATCTGCGG 3'

REV 5' TTGGCCTTGGTAATCCGCAC 3'

Fwd Tm 63.2°C

Rev Tm 62.8°C

GC 55%

Product 127 bp

Spans 1 intron

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