BEST3 60 primer pairs found

Human

Show all (including non-specific)

NM_152439 · chr12

117 1 gene · 4 transcripts

FWD 5' TTCCGCTGGTTTACACCCAG 3'

REV 5' TGTGCATTTCGTCCACAGCT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 132 bp

Spans 2 introns

NM_152439 · chr12

116 1 gene · 4 transcripts

FWD 5' ATGACTGGGTTGGGATTCCG 3'

REV 5' TGTGCATTTCGTCCACAGCT 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 147 bp

Spans 2 introns

NM_152439 · chr12

116 Specific

FWD 5' TACACCCAGGTAGCAGAGCA 3'

REV 5' TGTGCATTTCGTCCACAGCT 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 121 bp

Spans 2 introns

NM_152439 · chr12

115 Specific

FWD 5' CGCTGGTTTACACCCAGGTA 3'

REV 5' TGTGCATTTCGTCCACAGCT 3'

Fwd Tm 61.6°C

Rev Tm 62.1°C

GC 55%

Product 129 bp

Spans 2 introns

NM_152439 · chr12

114 1 gene · 4 transcripts

FWD 5' GGTTGGGATTCCGCTGGTTT 3'

REV 5' TGTGCATTTCGTCCACAGCT 3'

Fwd Tm 62.8°C

Rev Tm 62.1°C

GC 55%

Product 140 bp

Spans 2 introns

NM_152439 · chr12

113 Specific

FWD 5' TTACACCCAGGTAGCAGAGC 3'

REV 5' ATGTGCATTTCGTCCACAGC 3'

Fwd Tm 61.1°C

Rev Tm 60.9°C

GC 55%

Product 123 bp

Spans 2 introns

NM_152439 · chr12

113 Specific

FWD 5' CCAGGTAGCAGAGCAGCTTA 3'

REV 5' ATGTGCATTTCGTCCACAGC 3'

Fwd Tm 61.1°C

Rev Tm 60.9°C

GC 55%

Product 117 bp

Spans 2 introns

NM_152439 · chr12

113 Specific

FWD 5' CCAGGTAGCAGAGCAGCTTA 3'

REV 5' TGTGCATTTCGTCCACAGCT 3'

Fwd Tm 61.1°C

Rev Tm 62.1°C

GC 55%

Product 116 bp

Spans 2 introns

NM_152439 · chr12

112 Specific

FWD 5' CGCTGGTTTACACCCAGGTA 3'

REV 5' ATGTGCATTTCGTCCACAGC 3'

Fwd Tm 61.6°C

Rev Tm 60.9°C

GC 55%

Product 130 bp

Spans 2 introns

NM_152439 · chr12

112 Specific

FWD 5' TTACACCCAGGTAGCAGAGC 3'

REV 5' TGTGCATTTCGTCCACAGCT 3'

Fwd Tm 61.1°C

Rev Tm 62.1°C

GC 55%

Product 122 bp

Spans 2 introns

NM_001282614 · chr12

111 1 gene · 3 transcripts

FWD 5' GCTTAAGGTAGCAGAGCAGC 3'

REV 5' AGCTCATGTGCATTTCGTCC 3'

Fwd Tm 60.4°C

Rev Tm 60.4°C

GC 55%

Product 125 bp

Spans 2 introns

NM_001282613 · chr12

111 1 gene · 3 transcripts

FWD 5' GCTTAAGGTAGCAGAGCAGC 3'

REV 5' AGCTCATGTGCATTTCGTCC 3'

Fwd Tm 60.4°C

Rev Tm 60.4°C

GC 55%

Product 125 bp

Spans 2 introns

NM_032735 · chr12

111 1 gene · 3 transcripts

FWD 5' GCTTAAGGTAGCAGAGCAGC 3'

REV 5' AGCTCATGTGCATTTCGTCC 3'

Fwd Tm 60.4°C

Rev Tm 60.4°C

GC 55%

Product 125 bp

Spans 2 introns

NM_001282614 · chr12

111 1 gene · 3 transcripts

FWD 5' GCTTAAGGTAGCAGAGCAGC 3'

REV 5' GCTCATGTGCATTTCGTCCA 3'

Fwd Tm 60.4°C

Rev Tm 60.6°C

GC 55%

Product 124 bp

Spans 2 introns

NM_001282613 · chr12

111 1 gene · 3 transcripts

FWD 5' GCTTAAGGTAGCAGAGCAGC 3'

REV 5' GCTCATGTGCATTTCGTCCA 3'

Fwd Tm 60.4°C

Rev Tm 60.6°C

GC 55%

Product 124 bp

Spans 2 introns

NM_032735 · chr12

111 1 gene · 3 transcripts

FWD 5' GCTTAAGGTAGCAGAGCAGC 3'

REV 5' GCTCATGTGCATTTCGTCCA 3'

Fwd Tm 60.4°C

Rev Tm 60.6°C

GC 55%

Product 124 bp

Spans 2 introns

NM_001282614 · chr12

110 1 gene · 3 transcripts

FWD 5' GCTTAAGGTAGCAGAGCAGC 3'

REV 5' ATGTGCATTTCGTCCACAGC 3'

Fwd Tm 60.4°C

Rev Tm 60.9°C

GC 55%

Product 120 bp

Spans 2 introns

NM_001282613 · chr12

110 1 gene · 3 transcripts

FWD 5' GCTTAAGGTAGCAGAGCAGC 3'

REV 5' ATGTGCATTTCGTCCACAGC 3'

Fwd Tm 60.4°C

Rev Tm 60.9°C

GC 55%

Product 120 bp

Spans 2 introns

NM_032735 · chr12

110 1 gene · 3 transcripts

FWD 5' GCTTAAGGTAGCAGAGCAGC 3'

REV 5' ATGTGCATTTCGTCCACAGC 3'

Fwd Tm 60.4°C

Rev Tm 60.9°C

GC 55%

Product 120 bp

Spans 2 introns

NM_001282614 · chr12

110 1 gene · 3 transcripts

FWD 5' GCAGGATGGCTTAAGGTAGC 3'

REV 5' AGCTCATGTGCATTTCGTCC 3'

Fwd Tm 60.2°C

Rev Tm 60.4°C

GC 55%

Product 133 bp

Spans 2 introns

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