BEST4 10 primer pairs found

Human

Show all (including non-specific)

NM_153274 · chr1

118 Specific

FWD 5' CTTCTATGCTGGCTGGCTCA 3'

REV 5' TTTCGTCCACGGATAGCAGG 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 128 bp

Spans 2 introns

NM_153274 · chr1

118 Specific

FWD 5' TCTATGCTGGCTGGCTCAAG 3'

REV 5' TTTCGTCCACGGATAGCAGG 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 126 bp

Spans 2 introns

NM_153274 · chr1

118 Specific

FWD 5' TCACCACTCTGCTGCAGTTC 3'

REV 5' TTTCGTCCACGGATAGCAGG 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 150 bp

Spans 2 introns

NM_153274 · chr1

118 Specific

FWD 5' TCTTCTATGCTGGCTGGCTC 3'

REV 5' TTTCGTCCACGGATAGCAGG 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 129 bp

Spans 2 introns

NM_153274 · chr1

118 Specific

FWD 5' CACCACTCTGCTGCAGTTCT 3'

REV 5' TTTCGTCCACGGATAGCAGG 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 149 bp

Spans 2 introns

NM_153274 · chr1

117 Specific

FWD 5' TGCTTAGCATCACCTACCGG 3'

REV 5' GGTTCACCACGAGAGTCACA 3'

Fwd Tm 61.4°C

Rev Tm 61.4°C

GC 55%

Product 141 bp

Spans 2 introns

NM_153274 · chr1

117 Specific

FWD 5' TTAGCATCACCTACCGGCTG 3'

REV 5' GGTTCACCACGAGAGTCACA 3'

Fwd Tm 61.4°C

Rev Tm 61.4°C

GC 55%

Product 138 bp

Spans 2 introns

NM_153274 · chr1

117 Specific

FWD 5' GCTCAAGGTGGCTGAACAGA 3'

REV 5' TTTCGTCCACGGATAGCAGG 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 113 bp

Spans 2 introns

NM_153274 · chr1

116 Specific

FWD 5' CTTAGCATCACCTACCGGCT 3'

REV 5' GGTTCACCACGAGAGTCACA 3'

Fwd Tm 61.1°C

Rev Tm 61.4°C

GC 55%

Product 139 bp

Spans 2 introns

NM_153274 · chr1

116 Specific

FWD 5' CGCTGTGCTTAGCATCACCT 3'

REV 5' CGGTTCACCACGAGAGTCAC 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 147 bp

Spans 2 introns